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Team:Calgary/Project/DataPage
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| - | TITLE=Building a Naphthenic Acid Biosensor | + | TITLE=Building a Naphthenic Acid Biosensor| |
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<h2>The Design</h2> | <h2>The Design</h2> | ||
| - | </html>[[Image:UofC2011_DataPage1.png|thumb|600px|center|<b>Figure 1</b> In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since <i>E. coli</i> is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into <i>Pseudomonas</i> as well as protocols for use of our system in microalgae.<html> | + | </html>[[Image:UofC2011_DataPage1.png|thumb|600px|center|<b>Figure 1</b> In order to develop a naphthenic acid biosensor we were required to develop a sensory element which would respond specifically to this class of compounds. Additionally, we developed a novel electrochemical reporter system using the lacZ gene product Beta-galactosidase to detect and quantify the response of our promoter into a measurable system. Since <i>E. coli</i> is not naturally found in tailings ponds, we designed a novel conjugation system to transfer our reporter system into <i>Pseudomonas</i> as well as protocols for use of our system in microalgae.]]<html> |
</html> | </html> | ||
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Revision as of 21:25, 28 September 2011
The Project
Building a Naphthenic Acid Biosensor
The Design
