Team:Brown-Stanford/Lab/Protocols/ATCC1376

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(ATCC medium: 1376 Bacillus pasteurii NH4-YE medium)
 
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<html><h1>Protocol Code: M3</h1></html>
== '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' ==
== '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' ==
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*20.0 g Yeast extract
*20.0 g Yeast extract
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*10.0 g (NH<sub>4</sub>)2SO<sub>4</sub>
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*10.0 g (75.7 mM) (NH<sub>4</sub>)2SO<sub>4</sub>
*1.0 L 0.13 M Tris buffer (pH 9.0)
*1.0 L 0.13 M Tris buffer (pH 9.0)
*20.0 g Agar (if needed)
*20.0 g Agar (if needed)
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
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Latest revision as of 18:17, 28 September 2011

Brown-Stanford
iGEM

Protocol Code: M3

ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)

  • 20.0 g Yeast extract
  • 10.0 g (75.7 mM) (NH4)2SO4
  • 1.0 L 0.13 M Tris buffer (pH 9.0)
  • 20.0 g Agar (if needed)

Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.