Latest revision as of 18:17, 28 September 2011

REGObricks
Intro --------------------- ISRU --------------------- Biocement --------------------- S. pasteurii --------------------- Balloon
Flights --------------------- Transforming --------------------- Biobrick²
PowerCell
Intro --------------------- Cyanobacteria --------------------- Mars --------------------- Nutrients
FRETcetera
Intro --------------------- FRET etc. --------------------- Construct
Lab
Team --------------------- Parts --------------------- Notebook --------------------- Protocols --------------------- Safety
Partners
Collab --------------------- Sponsors
Outreach
SB5.0 --------------------- NASA --------------------- Lunar Science --------------------- Maker Faire --------------------- BBC --------------------- CBricks --------------------- Ethics --------------------- Regional
Jamboree

Protocol Code: M3

Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.

@@ Line 1: / Line 1: @@
-{{:Team:Brown-Stanford/Templates/Main}}
+{{:Team:Brown-Stanford/Templates/Protocol}}
+<html><h1>Protocol Code: M3</h1></html>
-== '''ATCC medium: 1376 ''Bacillus pasteurii'' NH4-YE medium''' ==
+== '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' ==
-.0 g Yeast extract
-.0 g (NH<sub>4</sub>)2SO<sub>4</sub>
-.0 L 0.13 M Tris buffer (pH 9.0)
-.0 g Agar (if needed)
-Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
+*20.0 g Yeast extract
+*10.0 g (75.7 mM) (NH<sub>4</sub>)2SO<sub>4</sub>
+*1.0 L 0.13 M Tris buffer (pH 9.0)
+*20.0 g Agar (if needed)
+Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.
 {{:Team:Brown-Stanford/Templates/Foot}}