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Team:Brown-Stanford/Lab/Protocols/BG11
From 2011.igem.org
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| - | {{:Team:Brown-Stanford/Templates/ | + | {{:Team:Brown-Stanford/Templates/Protocol}} |
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| + | <html><h1>Protocol Code: M1</h1></html> | ||
== '''BG-11''' == | == '''BG-11''' == | ||
| - | ''(Taken from | + | |
| + | ''(Taken from <html><a href=""http://microbiology.ucdavis.edu/meeks/BG11medium.html"">Meeks Lab at University of California, Davis</a></html>)'' | ||
=== '''Stock solutions for BG-11''' === | === '''Stock solutions for BG-11''' === | ||
| - | Stock 1 | + | ==== '''Stock 1''' ==== |
*0.1g/liter Na<sub>2</sub>MG EDTA | *0.1g/liter Na<sub>2</sub>MG EDTA | ||
*0.6g/liter Ferric ammonium citrate | *0.6g/liter Ferric ammonium citrate | ||
| Line 12: | Line 15: | ||
Filter sterilize into a sterile bottle or autoclave. | Filter sterilize into a sterile bottle or autoclave. | ||
| - | Stock 2 | + | ==== '''Stock 2''' ==== |
*7.5g/liter MgSO<sub>4</sub>•7H<sub>2</sub>O | *7.5g/liter MgSO<sub>4</sub>•7H<sub>2</sub>O | ||
Filter sterilize into a sterile bottle or autoclave. | Filter sterilize into a sterile bottle or autoclave. | ||
| - | Stock 3 | + | ==== '''Stock 3''' ==== |
| - | *4.0g/liter K<sub> | + | *4.0g/liter K<sub>2</sub>HPO<sub>4</sub>•3H<sub>2</sub>O or 3.05g/liter K<sub>2</sub>HPO<sub>4</sub> |
| - | + | ||
Filter sterilize into a sterile bottle or autoclave. | Filter sterilize into a sterile bottle or autoclave. | ||
| + | ==== '''Stock 5 (Microelements)''' ==== | ||
Stock 5 (Microelements): | Stock 5 (Microelements): | ||
*2.86g/liter H<sub>3</sub>BO<sub>3</sub> | *2.86g/liter H<sub>3</sub>BO<sub>3</sub> | ||
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*0.079g/liter CuSO<sub>4</sub>•5H<sub>2</sub>O | *0.079g/liter CuSO<sub>4</sub>•5H<sub>2</sub>O | ||
*0.050g/liter COCl<sub>2</sub>•6H<sub>2</sub>O | *0.050g/liter COCl<sub>2</sub>•6H<sub>2</sub>O | ||
| - | *0.391g/liter NaMoO<sub>4</sub>•2H<sub>2</sub>O | + | *0.391g/liter NaMoO<sub>4</sub>•2H<sub>2</sub>O or 0.018g/liter MoO<sub>4</sub> (85%) |
| - | + | ||
| - | + | ||
| - | + | === '''Basic BG11 medium''' === | |
| - | + | Combine the following stock solutions (for 1 liter): | |
*10 mL Stock 1 | *10 mL Stock 1 | ||
*10 mL Stock 2 | *10 mL Stock 2 | ||
| Line 40: | Line 41: | ||
*1.5 g NaNO<sub>3</sub> | *1.5 g NaNO<sub>3</sub> | ||
| - | Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 | + | Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO<sub>3</sub>. |
{{:Team:Brown-Stanford/Templates/Foot}} | {{:Team:Brown-Stanford/Templates/Foot}} | ||
Latest revision as of 18:15, 28 September 2011
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Protocol Code: M1
BG-11
(Taken from Meeks Lab at University of California, Davis)
Stock solutions for BG-11
Stock 1
- 0.1g/liter Na2MG EDTA
- 0.6g/liter Ferric ammonium citrate
- 0.6g/liter Citric acid•1H2O
- 3.6g/liter CaCl2•2H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 2
- 7.5g/liter MgSO4•7H2O
Filter sterilize into a sterile bottle or autoclave.
Stock 3
- 4.0g/liter K2HPO4•3H2O or 3.05g/liter K2HPO4
Filter sterilize into a sterile bottle or autoclave.
Stock 5 (Microelements)
Stock 5 (Microelements):
- 2.86g/liter H3BO3
- 1.81g/liter MnCl2•4H2O
- 0.222g/liter ZnSO4•7H2O
- 0.079g/liter CuSO4•5H2O
- 0.050g/liter COCl2•6H2O
- 0.391g/liter NaMoO4•2H2O or 0.018g/liter MoO4 (85%)
Basic BG11 medium
Combine the following stock solutions (for 1 liter):
- 10 mL Stock 1
- 10 mL Stock 2
- 10 mL Stock 3
- 0.02 g Na2CO3
- 1.0 mL Stock 5
- 1.5 g NaNO3
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 mL/125 mL flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11, don't add NaNO3.
