Team:Brown-Stanford/Lab/Notebook/Week0

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== ''' June 20, 2011''' ==
 
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*Made solid, liquid LB
 
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*Made kan, amp solid LB plates
 
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*made CCMB80 Buffer
 
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*Made BG11 Plates
 
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*Streaked out Golden strains on BG11 plates (in the 30? incubator)
 
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*Created 5mL liquid BG11 cultures of all the Golden strains (in the 30? incubator)
 
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*Streaked Norman’s E. coli conjugative plasmid strains on ampicilin to verify plasmid retention
 
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== ''' June 21, 2011''' ==
 
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*Designed construct (see construct page)
 
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*Designed Co-culture experiment (see experiment design page)
 
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*Incubate with shaking (110rpm) at 30?
 
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*Max created liquid culture of Norman’s RP1 strain
 
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== ''' June 22, 2011''' ==
 
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Oops, shouldn’t have incubated with shaking.  Without shaking until green, then move to shaking at 110rpm.  Took cyano liquid cultures off shaking platform.
 
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Created liquid culture of Silver’s sucrose invertase/glucose export S. elongatus strain in BG11
 
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Created stocks for plasmid prep
 
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== ''' June 23, 2011''' ==
 
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Made BG11 stocks except Na2CO3
 
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Made 50mg/mL streptomycin stock
 
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Plasmid prepped Norman’s strains using Norman’s plasmid prep protocol:
 
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*D1: pUC18@DH5a NW20110620.41
 
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*D2: pSB1A3-P1010@DB3.1 NW20110620.A2
 
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*D3: pSB1A10-P1010@DB3.1 NW20110620.A3
 
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*D4: pSB1A10-J133452@DH10B NW20110620.A4
 
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*E1: RP1@HB101 EM20110620.A1-
 
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*E2: PSB3k3-P1010@DB3.1 EM20110620.A2
 
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*E3: PSB3k5-P1010@DB3.1 EM20110620.A3
 
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*E4: PSB3k5m(>-)-P1010 DB3.1 EM20110620.A4-
 
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-During plasmid prep, 1ml of Sol2 (instead of 300ul) added; user spun down samples, removed some of Sol2, before adding Sol3; asterisked samples already had some Sol3 added before spinning and removing (therefore some of the cells may have already lysed, the DNA was poured off)
 
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Finalized construct design (see construct page)
 
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== ''' June 24, 2011''' ==
 
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Transferred cyanos to 10ml cultures in square flasks
 
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created Na2CO3 stock
 
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cryostocked norman’s plasmids (see 6/23/11)
 
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Silver’s S. elongatus isn’t doing so well
 
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Learned about gibson assembly, designed primers for constructs
 
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Revision as of 22:52, 25 September 2011

Brown-Stanford
iGEM


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