Team:Brown-Stanford/Lab/Protocols/ATCC1376

From 2011.igem.org

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(ATCC medium: 1376 Bacillus pasteurii NH4-YE medium)
(ATCC medium: 1376 Bacillus pasteurii NH4-YE medium)
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== '''ATCC medium: 1376 ''Bacillus pasteurii'' NH<sub>4</sub>-YE medium''' ==
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== '''ATCC medium: 1376 (''Bacillus pasteurii'' NH<sub>4</sub>-YE medium)''' ==
*20.0 g Yeast extract
*20.0 g Yeast extract
*10.0 g (NH<sub>4</sub>)2SO<sub>4</sub>
*10.0 g (NH<sub>4</sub>)2SO<sub>4</sub>

Revision as of 23:13, 11 June 2011

Brown-Stanford
iGEM

ATCC medium: 1376 (Bacillus pasteurii NH4-YE medium)

  • 20.0 g Yeast extract
  • 10.0 g (NH4)2SO4
  • 1.0 L 0.13 M Tris buffer (pH 9.0)
  • 20.0 g Agar (if needed)

Autoclave ingredients separately. No growth occurs when ingredients are sterilized together.