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18 (May 18, 2011 GDS)
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Parth--made fresh TENS | Parth--made fresh TENS | ||
| - | ::4.5 TE | + | :::4.5 TE |
| - | ::250λ 10% SDS | + | :::250λ 10% SDS |
| - | ::250λ 2M NaOH | + | :::250λ 2M NaOH |
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
::Spin 1.5 mL (2 tubes) 30" | ::Spin 1.5 mL (2 tubes) 30" | ||
| Line 19: | Line 14: | ||
::Spin 3' ________ | ::Spin 3' ________ | ||
::Wash 70% ETOH | ::Wash 70% ETOH | ||
| + | |||
| + | |||
| + | |||
| + | :Minipreps (2 each) for: | ||
| + | :pNICE SA-15λ TE | ||
| + | :J024430 GE-15λ TE | ||
| + | :Trans002-5, AB-Resp 30&lambda | ||
| + | :Trans003-W1 SD, SA- | ||
Revision as of 19:18, 5 September 2011
Parth--made fresh TENS
- 4.5 TE
- 250λ 10% SDS
- 250λ 2M NaOH
- Spin 1.5 mL (2 tubes) 30"
- Pour off sol'n + resuspend
- Add: 300λ TENS mix
- 150λ NaOAc mix
- Spin 2.5'
- Sup to new tube
- Add 1 mL ETOH - mix well
- Spin 3' ________
- Wash 70% ETOH
- Minipreps (2 each) for:
- pNICE SA-15λ TE
- J024430 GE-15λ TE
- Trans002-5, AB-Resp 30&lambda
- Trans003-W1 SD, SA-
