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From 2011.igem.org

Revision as of 20:01, 4 September 2011 (view source) Sallen (Talk | contribs) ← Older edit		Revision as of 20:02, 4 September 2011 (view source) Sallen (Talk | contribs) Newer edit →
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	::Spin 1.5 mL (2 tubes) 30"		::Spin 1.5 mL (2 tubes) 30"
	::Pour off sol'n + resuspend		::Pour off sol'n + resuspend
-	::Add: 300&lambda TENS mix	+	::Add: 300λ TENS mix
-	::    150&lambda NaOAc mix	+	:::    150λ NaOAc mix
	::Spin 2.5'		::Spin 2.5'
	::Sup to new tube		::Sup to new tube
	::Add 1 mL ETOH - mix well		::Add 1 mL ETOH - mix well
	::Spin 3' ________		::Spin 3' ________
-	Wash 70% ETOH	+	::Wash 70% ETOH

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Revision as of 20:02, 4 September 2011

Parth--made fresh TENS

4.5 TE

250λ 10% SDS

250λ 2M NaOH

Minipreps (2 each) for:

pNICE SA-15λ TE

J024430 GE-15λ TE

Trans002-5, AB-Resp 30&lambda

Trans003-W1 SD, SA-

Spin 1.5 mL (2 tubes) 30"

Pour off sol'n + resuspend

Add: 300λ TENS mix

150λ NaOAc mix

Spin 2.5'

Sup to new tube

Add 1 mL ETOH - mix well

Spin 3' ________

Wash 70% ETOH