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18 (May 18, 2011 GDS)
From 2011.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
::250λ 10% SDS | ::250λ 10% SDS | ||
::250λ 2M NaOH | ::250λ 2M NaOH | ||
| + | |||
| + | :Minipreps (2 each) for: | ||
| + | :pNICE SA-15λ TE | ||
| + | :J024430 GE-15λ TE | ||
| + | :Trans002-5, AB-Resp 30&lambda | ||
| + | :Trans003-W1 SD, SA- | ||
| + | |||
| + | ::Spin 1.5 mL (2 tubes) 30" | ||
| + | ::Pour off sol'n + resuspend | ||
| + | ::Add: 300&lambda TENS mix | ||
| + | :: 150&lambda NaOAc mix | ||
| + | ::Spin 2.5' | ||
| + | ::Sup to new tube | ||
| + | ::Add 1 mL ETOH - mix well | ||
| + | ::Spin 3' ________ | ||
| + | Wash 70% ETOH | ||
Revision as of 20:01, 4 September 2011
Parth--made fresh TENS
- 4.5 TE
- 250λ 10% SDS
- 250λ 2M NaOH
- Minipreps (2 each) for:
- pNICE SA-15λ TE
- J024430 GE-15λ TE
- Trans002-5, AB-Resp 30&lambda
- Trans003-W1 SD, SA-
- Spin 1.5 mL (2 tubes) 30"
- Pour off sol'n + resuspend
- Add: 300&lambda TENS mix
- 150&lambda NaOAc mix
- Spin 2.5'
- Sup to new tube
- Add 1 mL ETOH - mix well
- Spin 3' ________
Wash 70% ETOH
