Team:UT Dallas/week9

Week 9

8/28/11	Transformation (DH5-alpha): GFP-RBS-TetR-term Incubation of CheZ, CheZ*

8/29/11	Glycerol/Miniprep ctx-CheZ, ctx-CheZ* Digestion: ToxR, FGFR Incubation ToxR

8/30/11	Test Digestion: ctx-CheZ, ctx-Chez, CheZ, CheZ Gel Electrophoresis: ctx-CheZ, ctx-Chez* Incubation: ToxR

8/31/11	Miniprep: ToxR Full Digestion: ToxR, pSB1C3 Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ, FGFR, RBS-TetR-Term Transformation: Ctx-CheZ, ToxR-FGFR, GFP-RBS-TetR-Term

9/1/11	Plating: k422007 CheY Incubation: GFP-RBS-TetR-Term (Carb) Incubation: E.Coli FHK12 - from Germany (Carb) Incubation: CheZ* (Chlora)

9/2/11	Plates Made: CarbR, ChloraR, CarbR/ChloraR Broth Made: LB CarbR, LB Carb/ChloraR Miniprep: GFP-RBS-TetR-Term, CheZ* Glycerol Stock: GFP-RBS-TetR-Term Made Competent Cells: FHK12 (Carb) Test Digestion/Gel Electrophoresis: GFP-RBS-TetR-Term, Full Digestion: CheZ, FGFR Ful Digestion: GFP-RBS-TetR-Term Transformation: German Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR Plasmid stock: Diluted German parts (as above) Ligation 10 Min: ctx, ToxR into CheZ, FGFR respectively Transformation: CheZ, ctx-CheZ, FGFR, ToxR-FGFR Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp Inserts= ctx, GFP-RBS, TetR-Term

9/3/11	Transformation of the followings: ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term