"

From 2011.igem.org

OmpA-sh3 parts

 draw line
 pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate
  --Zora 02:54, 2 September 2011 (CDT)

new plasmid

 digest   
 pSB1C3 5ug
 digest with NEB EcoRI&SpeI
 --Zora 02:54, 2 September 2011 (CDT)

pet15a - OHS parts

new plasmid

  gel analysis
  OmpA-HIV cleavage site-Sh3 digested done by ZLY
  pSB1C3 digested
  
 
  gel extraction
  OHS vector Conc. 38ng/ul
  pSB1C3 vector Conc. 55ng/ul
  --Zora 01:28, 2 September 2011 (CDT)