Team:Paris Liliane Bettencourt/Notebook/2011/07/03/

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Mathias & Laura

IPTG inducible expression BioBrick

Miniprep

-LacI K143033

-Standard Terminator B0015

Digestion and gel extraction

-LacI by EcoR1 and Spe1

-Standard Terminator by EcoR1 and Xba1

Ligation and transformation

-LacI & Standard Terminator => LacI + Term