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From 2011.igem.org

We prepared:

 500ml LB medium

 Autoclaving LB medium

 Glycerol 30% for preservation

 Check the medium with plasmids, colonies grows well; negative control doesn’t grow after 22h on incubation.

 We inoculate bacterias with plasmids P+R, R+T and with Rh in liquid LB + Amp. Incubate at 37oC in shaker.