Team:Osaka/week6

September 4 (Sun)

1. Restriction digests:3-10C,1-2M
2. Gel extraction of digests
3. Ligation
   • 03:1-9N+3-6H again
4. Transformation of ligation products
5. Photospectrometry measuament

September 5 (Mon)

1. Absorbance measurement
2. To liquid culture:03,17,3-10C

September 6 (Tue)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
   • all parts ×
4. PCR to amplify PprI,PprA,PprM,RecA (DNA repair protein) from D.radiodurance
5. Gel Electrophoresis of PCR products
   • PprM ok
   • PprI,PprA,RecA ×
6. PCR
7. Gel Electrophoresis of PCR products
   • ×

September 7 (Wed)

1. PCR
2. Gel electrophoresis of PCR products
   • PprM ok
   • PprI,PprA,RecA ×
3. Gel electrophoresis of PprM
4. Gel extraction
5. PCR
6. Gel electrophoresis of PCR products
   • PprI,PprA,RecA ×
7. Miniprep of yesterday's culture
8. Restriction digests of miniprepped parts
9. Gel electrophoresis of digests
   • 03 ok
10. To liquid culture:03,04 (preincubation)
11. PCR
12. Electrophoresis of PCR products
13. PCR

September 8 (Thu)

1. Electrophoresis of PCR products
2. Restriction digests of 1-3A(backbone)
3. PCR
4. Electrophoresis of PCR products and miniprepped parts
5. Gel extraction of 1-3A
6. PCR
7. Electrophoresis of PCR products
8. Inoculation of 03,04
9. To liquid culture 03,04
10. PCR
11. Electrophoresis of PCR products

September 9 (Fri)

1. PCR
2. Electrophoresis of PCR products
3. Gel extraction
4. Restriction digests
5. PCR
6. Electrophoresis of PCR products
7. Gel extraction
8. Restriction digests
9. Ligation
   • 101(C):PprI+1-3A(vector)
   • 102(C):PprA+1-3A(vector)
10. Transformation of ligation products
11. Absorbance measurement

September 10 (Sat)

1. PCR
2. Electrophoresis of PCR products
3. To liquid culture:101,102
4. PCR
5. PCR purification
6. Absorbance measurement

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