http://2011.igem.org/wiki/index.php?title=Team:XMU-China/Partner&feed=atom&action=historyTeam:XMU-China/Partner - Revision history2024-03-29T07:25:18ZRevision history for this page on the wikiMediaWiki 1.16.0http://2011.igem.org/wiki/index.php?title=Team:XMU-China/Partner&diff=234153&oldid=prevXmuwendy at 01:17, 6 October 20112011-10-06T01:17:38Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We want to thank Professor Baishan Fang for providing us all the facilities and materials needed in the experiments and for the financial support. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We <ins class="diffchange diffchange-inline">want </ins>to <ins class="diffchange diffchange-inline">thank Yongzhen Hou </ins>from <ins class="diffchange diffchange-inline">Beijing University </ins>of <ins class="diffchange diffchange-inline">Posts and Telecommunications who support us </ins>in the <ins class="diffchange diffchange-inline">wiki construction</ins>.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">== '''Project Name''' ==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">i-''ccdB'': intelligent Control of Cell Density in Bacteria</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">== Project Abstract==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We <del class="diffchange diffchange-inline">have developed a series of devices which program a bacteria population </del>to <del class="diffchange diffchange-inline">maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in ''E. coli'' based on the well-known quorum-sensing system </del>from <del class="diffchange diffchange-inline">''Vibrio fischeri'', which autonomously regulates the density </del>of <del class="diffchange diffchange-inline">an ''E. coli'' population. The cell density however is influenced by the expression levels of a killer gene (''ccdB'') </del>in <del class="diffchange diffchange-inline">our device. As such, we have successfully controlled </del>the <del class="diffchange diffchange-inline">expression levels of ''ccdB'' by site-directed mutagenesis of a ''luxR'' promoter (''lux pr'') and error-prone PCR of gene ''luxR'', and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an ''E. coli'' population. This work can serve as a foundation for future advances involving fermentation industry and information processing</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=== Backgrounds ===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=== Project Detail===</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">== Results ==</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Xmuwendyhttp://2011.igem.org/wiki/index.php?title=Team:XMU-China/Partner&diff=232123&oldid=prevXmuwendy at 21:26, 5 October 20112011-10-05T21:26:55Z<p></p>
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</table>Xmuwendyhttp://2011.igem.org/wiki/index.php?title=Team:XMU-China/Partner&diff=218363&oldid=prev3Zleader: Created page with "<html> <style type="text/css"> #gTop { width: 975px; height: 806px; margin: 0 auto 0 auto; border:none; position:relative; background:url(https://static.igem.org/mediawiki/2011..."2011-10-04T15:59:47Z<p>Created page with "<html> <style type="text/css"> #gTop { width: 975px; height: 806px; margin: 0 auto 0 auto; border:none; position:relative; background:url(https://static.igem.org/mediawiki/2011..."</p>
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== '''Project Name''' ==<br />
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i-''ccdB'': intelligent Control of Cell Density in Bacteria<br />
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We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in ''E. coli'' based on the well-known quorum-sensing system from ''Vibrio fischeri'', which autonomously regulates the density of an ''E. coli'' population. The cell density however is influenced by the expression levels of a killer gene (''ccdB'') in our device. As such, we have successfully controlled the expression levels of ''ccdB'' by site-directed mutagenesis of a ''luxR'' promoter (''lux pr'') and error-prone PCR of gene ''luxR'', and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an ''E. coli'' population. This work can serve as a foundation for future advances involving fermentation industry and information processing.<br />
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=== Backgrounds ===<br />
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