Team:XMU-China/Notebook

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Revision as of 11:44, 7 September 2011

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Notebook

2011-08-05

We found the plasmids in the second generation bl21 in wrong sizes after long time reservation in -20℃. Our team leader went crazy.

2011-09-02

The curve diagram of quantity and that of fluorescence seemed strange. We were confused and frustrated. Through discussions and seeking others for help, we sorted out some reasons that might lead to the failure. It was planned that the experiment would be done another time next week. Methods of dealing with samples will be changed and details in every step will be attached importance to. We expect a good result of the coming week. Cheer up!

2011-09-05

We added our first biobrick part:BBa_K658000 in the registry.What a nice day!

2011-09-07

We made up the solid culture media in preparation for getting the growth curve of BL21 with the population-control device and BL21 bacteria as a control group. When the OD of the inoculated bacteria reached the expected value,the first sampling was made.The conical flask with the bacteria was put back in the bacterial culture shaker for growth.It was planned to take samples every hour in the first 8 hours.

@@ Line 31: / Line 31: @@
 ===2011-09-05===
 We added our first biobrick part:BBa_K658000 in the registry.What a nice day!
+===2011-09-07===
+We made up the solid culture media in preparation for getting the growth curve of BL21 with the population-control device and BL21 bacteria as a control group.
+When the OD of the inoculated bacteria reached the expected value,the first sampling was made.The conical flask with the bacteria was put back in the bacterial culture shaker for growth.It was planned to take samples every hour in the first 8 hours.