Team:XMU-China/Labjournal
From 2011.igem.org
(Difference between revisions)
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(We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! ) | (We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! ) | ||
- | + | [[Image:XMU_China_150.jpg|left]] | |
+ | |||
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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===Week 2(10th Apr.—16th Apr.)=== | ===Week 2(10th Apr.—16th Apr.)=== | ||
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Constructing the BioBrick H. The figure shown below and sequence analysis can indicate that it is correctly constructed. | Constructing the BioBrick H. The figure shown below and sequence analysis can indicate that it is correctly constructed. | ||
- | + | [[Image:XMU_China_151.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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===Week 4(24th Apr.—30th Apr.)=== | ===Week 4(24th Apr.—30th Apr.)=== | ||
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Testing on the performance of the part using different concentrations of AHL as inducer.The result is shown as follows: | Testing on the performance of the part using different concentrations of AHL as inducer.The result is shown as follows: | ||
- | + | [[Image:XMU_China_152.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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===Week 5-8(1st May—28th May)=== | ===Week 5-8(1st May—28th May)=== | ||
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Constructing the population-control device with RBS0.07 | Constructing the population-control device with RBS0.07 | ||
- | + | [[Image:XMU_China_153.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | ||
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Constructing the population-control device with RBS1.0 | Constructing the population-control device with RBS1.0 | ||
- | + | [[Image:XMU_China_154.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT; | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT; | ||
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Constructing the population-control device with RBS0.3 | Constructing the population-control device with RBS0.3 | ||
- | + | [[Image:XMU_China_155.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | ||
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Conducting the experiment on the cell growth of the two devices with RBS0.07. | Conducting the experiment on the cell growth of the two devices with RBS0.07. | ||
- | + | [[Image:XMU_China_156.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
Conducting the experiment on the viable cell density at steady state of the four devices with RBS0.07, RBS0.3, RBS0.6 and RBS1.0 | Conducting the experiment on the viable cell density at steady state of the four devices with RBS0.07, RBS0.3, RBS0.6 and RBS1.0 | ||
- | + | [[Image:XMU_China_157.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
===Week 9-17(29th May—30th Jul.)=== | ===Week 9-17(29th May—30th Jul.)=== | ||
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Proving the three mutants wrong which is shown as follows: | Proving the three mutants wrong which is shown as follows: | ||
- | + | [[Image:XMU_China_158.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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By restriction analysis, the restored IR-gfp identified to be broken | By restriction analysis, the restored IR-gfp identified to be broken | ||
- | + | [[Image:XMU_China_159.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
Reconstructing IR-gfp | Reconstructing IR-gfp | ||
- | + | [[Image:XMU_China_160.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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Successfully getting the mutant at site 3, site 5 and site 3/5 which can be verified by sequence analysis | Successfully getting the mutant at site 3, site 5 and site 3/5 which can be verified by sequence analysis | ||
- | + | [[Image:XMU_China_161.jpg|left]] | |
+ | |||
+ | [[Image:XMU_China_162.jpg|left]] | ||
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+ | [[Image:XMU_China_163.jpg|left]] | ||
- | |||
+ | [[Image:XMU_China_164.jpg|left]] | ||
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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- | + | [[Image:XMU_China_165.jpg|left]] | |
- | + | [[Image:XMU_China_166.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
===Week 18-21(31st Jul.—27th Aug.)=== | ===Week 18-21(31st Jul.—27th Aug.)=== | ||
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- | + | [[Image:XMU_China_167.jpg|left]] | |
+ | |||
+ | [[Image:XMU_China_168.jpg|left]] | ||
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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Successfully getting the mutant H3 | Successfully getting the mutant H3 | ||
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- | + | [[Image:XMU_China_169.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
+ | |||
===Week 20-22(28th Aug.—17th Sep.)=== | ===Week 20-22(28th Aug.—17th Sep.)=== | ||
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- | + | [[Image:XMU_China_170.jpg|left]] | |
- | + | [[Image:XMU_China_171.jpg|left]] | |
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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- | + | [[Image:XMU_China_172.jpg|left]] | |
+ | |||
+ | [[Image:XMU_China_173.jpg|left]] | ||
+ | <html> | ||
+ | <img src="http://partsregistry.org/wiki/images/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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===Week 23(18th Sep.—24th Sep.)=== | ===Week 23(18th Sep.—24th Sep.)=== | ||
Changing the backbone of the plasmids according to the requirement | Changing the backbone of the plasmids according to the requirement |
Latest revision as of 01:43, 6 October 2011