Team:XMU-China/Labjournal
From 2011.igem.org
(Difference between revisions)
(→Wetlab journal) |
|||
Line 16: | Line 16: | ||
(We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! ) | (We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! ) | ||
- | Fig. | + | XXXXXXXXXXXXXX Fig.150 XXXXXXXXXXXXXXXXXXXX |
===Week 2(10th Apr.—16th Apr.)=== | ===Week 2(10th Apr.—16th Apr.)=== | ||
Line 36: | Line 36: | ||
Constructing the BioBrick H. The figure shown below and sequence analysis can indicate that it is correctly constructed. | Constructing the BioBrick H. The figure shown below and sequence analysis can indicate that it is correctly constructed. | ||
- | Fig. | + | XXXXXXXXXXXXXXXXX Fig.151 XXXXXXXXXXXXXXXXXX |
===Week 4(24th Apr.—30th Apr.)=== | ===Week 4(24th Apr.—30th Apr.)=== | ||
Line 47: | Line 47: | ||
Testing on the performance of the part using different concentrations of AHL as inducer.The result is shown as follows: | Testing on the performance of the part using different concentrations of AHL as inducer.The result is shown as follows: | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXX Fig.152 XXXXXXXXXXXXXXXXXX |
===Week 5-8(1st May—28th May)=== | ===Week 5-8(1st May—28th May)=== | ||
Line 60: | Line 60: | ||
Constructing the population-control device with RBS0.07 | Constructing the population-control device with RBS0.07 | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXX Fig.153 XXXXXXXXXXXXXXXXXX |
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | ||
Line 89: | Line 89: | ||
Constructing the population-control device with RBS1.0 | Constructing the population-control device with RBS1.0 | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXX Fig.154 XXXXXXXXXXXXXXXXXXXXXXXXX |
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT; | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT; | ||
Line 118: | Line 118: | ||
Constructing the population-control device with RBS0.3 | Constructing the population-control device with RBS0.3 | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.155 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | S1: single endonuclease digestion of pSB1A2-PlacO-1-RBS1.0-luxI-TT | ||
Line 150: | Line 150: | ||
Conducting the experiment on the cell growth of the two devices with RBS0.07. | Conducting the experiment on the cell growth of the two devices with RBS0.07. | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.156 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
Conducting the experiment on the viable cell density at steady state of the four devices with RBS0.07, RBS0.3, RBS0.6 and RBS1.0 | Conducting the experiment on the viable cell density at steady state of the four devices with RBS0.07, RBS0.3, RBS0.6 and RBS1.0 | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.157 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
===Week 9-17(29th May—30th Jul.)=== | ===Week 9-17(29th May—30th Jul.)=== | ||
Line 189: | Line 189: | ||
Proving the three mutants wrong which is shown as follows: | Proving the three mutants wrong which is shown as follows: | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.158 XXXXXXXXXXXXXXXXXXXXXXXXXX |
Line 196: | Line 196: | ||
By restriction analysis, the restored IR-gfp identified to be broken | By restriction analysis, the restored IR-gfp identified to be broken | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.159 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
Reconstructing IR-gfp | Reconstructing IR-gfp | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.160 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
Line 207: | Line 207: | ||
Successfully getting the mutant at site 3, site 5 and site 3/5 which can be verified by sequence analysis | Successfully getting the mutant at site 3, site 5 and site 3/5 which can be verified by sequence analysis | ||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.161 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.162 XXXXXXXXXXXXXXXXXXXXXXXXXXXX |
- | |||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.163 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
+ | |||
+ | |||
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.164 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
Line 220: | Line 222: | ||
Conducting the experiment of determining the fluorescence curve of the three devices with mutations respectively at site 3, 5, 3/5 | Conducting the experiment of determining the fluorescence curve of the three devices with mutations respectively at site 3, 5, 3/5 | ||
- | |||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.165XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
+ | |||
+ | |||
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.166 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
===Week 18-21(31st Jul.—27th Aug.)=== | ===Week 18-21(31st Jul.—27th Aug.)=== | ||
Line 234: | Line 238: | ||
Activation of H for the following experiment | Activation of H for the following experiment | ||
- | |||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.167 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
+ | |||
+ | |||
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.168 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
Successfully getting the mutant H3 | Successfully getting the mutant H3 | ||
Line 252: | Line 258: | ||
Conducting the experiment of the bacteria quantity of the population-control device with mutations respectively at site 3, 5, 3/5 | Conducting the experiment of the bacteria quantity of the population-control device with mutations respectively at site 3, 5, 3/5 | ||
- | Fig. | + | |
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.169 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
===Week 20-22(28th Aug.—17th Sep.)=== | ===Week 20-22(28th Aug.—17th Sep.)=== | ||
Line 268: | Line 275: | ||
- | |||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.170 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
+ | |||
+ | |||
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.171 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
Line 284: | Line 293: | ||
Repeating and optimizing the work done last week to get better results. | Repeating and optimizing the work done last week to get better results. | ||
- | |||
- | Fig. | + | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.172 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX |
+ | |||
+ | |||
+ | XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Fig.173 XXXXXXXXXXXXXXXXXXXXXXXXXXXXX | ||
===Week 23(18th Sep.—24th Sep.)=== | ===Week 23(18th Sep.—24th Sep.)=== | ||
Changing the backbone of the plasmids according to the requirement | Changing the backbone of the plasmids according to the requirement |
Revision as of 00:05, 6 October 2011