Team:XMU-China/Data

From 2011.igem.org

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== How does i-''ccdB'' work==
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== '''Data for favorite new parts''' ==
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=== Backgrounds ===
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[BBa_K658003]http://partsregistry.org/wiki/index.php?title=Part:BBa_K658003 --a bacteria population-control device with RBS0.6 driven by lacl+pL
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=== Project Detail===
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The device is designed to build a programmed bacterial death circuit.Based on the device,a series of bacteria population-control devices (BBa_K658001,BBa_K658004,BBa_K658005) are designed using RBSs of different strength in the killer protein producer. Also, we have designed a series of population-control devices(BBa_K658010,BBa_K658011,BBa_K658012) by mutagenesis at positition 3,5and 3/5 of lux pR.
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A comparison in performance of all the population-control devices can be found in the experience page.
== Results ==
== Results ==

Revision as of 12:53, 5 October 2011


Home Team Project Model Result Data Safety Human Practice Partner Description Approach Background Protocols Wetlab Journal



How our devices work

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Figure 1: The synthesis of LuxR protein and the signalling molecule N-acyl-homoserine lactone (AHL) is induced by adding Isopropyl β-D-1-thiogalactopyranoside (IPTG). The AHL accumulates in the experimental medium and inside the cells as the cell density increases. At sufficiently high concentrations, it binds and activates the LuxR transcriptional regulator, which in turn induces the expression of a killer gene ccdB under the control of a promoter lux pR. Sufficiently high levels of the killer protein cause cell death. This circuit programmes a bacterial population to maintain a cell density that is lower than the limits imposed by the environment.

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Figure 2:

Data for favorite new parts

[BBa_K658003]http://partsregistry.org/wiki/index.php?title=Part:BBa_K658003 --a bacteria population-control device with RBS0.6 driven by lacl+pL

The device is designed to build a programmed bacterial death circuit.Based on the device,a series of bacteria population-control devices (BBa_K658001,BBa_K658004,BBa_K658005) are designed using RBSs of different strength in the killer protein producer. Also, we have designed a series of population-control devices(BBa_K658010,BBa_K658011,BBa_K658012) by mutagenesis at positition 3,5and 3/5 of lux pR.

A comparison in performance of all the population-control devices can be found in the experience page.

Results