Team:Washington/Team/Members

From 2011.igem.org

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== '''Who we are''' ==
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<gallery caption="Undergraduate Team Members" widths="180px" heights="120px" perrow="3">
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Image:Washington_jeff_johnson.png|<center>Jeff Johnson</center>
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Image:chris_eiben.jpg|<center>Chris Eiben</center>
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Image:Washington_Huber_Tomas.jpg|<center>Tomas Huber</center>
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Image:Washington_Ivan_Huang.jpg|<center>Ivan Huang</center>
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Image:Washington_Peixian.JPG‎|<center>Peixian Liu</center>
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Image:Washington_Denny_Luan.JPG|<center>Denny Luan</center>
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Image:Wu_Cindy.jpg|<center>Cindy Wu</center>
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Image:Washington_Sean_Wu.jpg|<center>Sean Wu</center>
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Image:Washington_david_zong.jpg‎|<center>David Zong</center>
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Image:Laura_Austin.jpg|<center>Laura Austin</center>
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Image:me2.jpg|<center>Matt Coyne</center>
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Image:Washington_Justin.jpg|<center>Justin De Leon</center>
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Image:Matthew_Harger.JPG|<center>Matthew Harger</center>
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Image:DSC06208.JPG|<center>Jesa Landis</center>
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</gallery>
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<gallery caption="Graduate Advisors" widths="180px" heights="120px" perrow="3">
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Image:Washington_matt_smith.png|<center>Matt Smith <br/> Molecular and Cellular Biology</center>
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Image:Ingrid.jpg|<center>Ingrid Swanson <br/> Microbiology <br/></center>
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Image:Regbert.jpg|<center>Rob Egbert <br/> Electrical Engineering</center>
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Image:galdzicki.jpg|<center>Michal Galdzicki <br/> Biomedical and Health Informatics</center>
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Image:Washington_Siegel_2010.jpg|<center>Justin Siegel <br/> Biomolecular Structure and Design</center>
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</gallery>
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<gallery caption="Faculty Advisors" widths="180px" heights="120px" perrow="3">
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image:Washington_David_Baker.jpg|<center>David Baker <br/> Biochemistry</center>
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Image:Washington_Klavins.jpg|<center>Eric Klavins <br/> Electrical Engineering</center>
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image:Washington_Mougous.jpg|<center>Joseph Mougous <br/> Microbiology</center>
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Image:Washington_Sauro.jpg|<center>Herbert Sauro <br/> Bioengineering</center>
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</gallery>
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<gallery caption="Collaborators and Support" widths="180px" heights="120px" perrow="3">
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Image:Washington_Biochemistry.gif|<center>Biochemistry <br/> Lab space</center>
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image:Washington_uwmb_header.jpg|<center>Microbiology <br/> Financial support and lab space</center>
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Image:Wahsington_Fisher_Logo.jpg|<center>Fisher <br/> Lab Materials</center>
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Image:Washington_Arthur_Friedlander.jpg|<center>Arthur Friedlander @ USAMRIID <br/> Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!</center>
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Image:Washington_Chandran.png|<center>Deepak Chandran @ Bioengineering, University of Washington<br/> Always available to answer our TinkerCell API questions no matter how trivial.</center>
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</gallery>
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== '''Who did what''' ==
 +
The teams were assembled during our winter quarter.  During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures.  During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out.  Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.
 +
 
 +
=== The Gram Positive Therapeutic ===
 +
Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.
 +
 
 +
=== The Gram Negative Therapeutic ===
 +
Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.
 +
 
 +
Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.
 +
 
 +
=== New Tools ===
 +
Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_68:_Standard_for_the_Electronic_Distribution_of_SBOLv_Diagrams BBF RFC 68]. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.
 +
 
 +
Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen.  He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.

Revision as of 06:02, 4 September 2011

Contents

Who we are

Undergraduate Team Members
Washington jeff johnson.png
Jeff Johnson
Chris eiben.jpg
Chris Eiben
Washington Huber Tomas.jpg
Tomas Huber
Washington Ivan Huang.jpg
Ivan Huang
Washington Peixian.JPG
Peixian Liu
Washington Denny Luan.JPG
Denny Luan
Wu Cindy.jpg
Cindy Wu
Sean Wu
David Zong
Laura Austin.jpg
Laura Austin
Me2.jpg
Matt Coyne
Washington Justin.jpg
Justin De Leon
Matthew Harger.JPG
Matthew Harger
DSC06208.JPG
Jesa Landis


Graduate Advisors
Washington matt smith.png
Matt Smith
Molecular and Cellular Biology
Ingrid.jpg
Ingrid Swanson
Microbiology
Rob Egbert
Electrical Engineering
Galdzicki.jpg
Michal Galdzicki
Biomedical and Health Informatics
Washington Siegel 2010.jpg
Justin Siegel
Biomolecular Structure and Design



Faculty Advisors
David Baker
Biochemistry
Eric Klavins
Electrical Engineering
Washington Mougous.jpg
Joseph Mougous
Microbiology
Washington Sauro.jpg
Herbert Sauro
Bioengineering


Collaborators and Support
Washington Biochemistry.gif
Biochemistry
Lab space
Washington uwmb header.jpg
Microbiology
Financial support and lab space
Wahsington Fisher Logo.jpg
Fisher
Lab Materials
Washington Arthur Friedlander.jpg
Arthur Friedlander @ USAMRIID
Help planning the project and offering to test our new enzymes' effectiveness against Anthrax!
Washington Chandran.png
Deepak Chandran @ Bioengineering, University of Washington
Always available to answer our TinkerCell API questions no matter how trivial.

Who did what

The teams were assembled during our winter quarter. During this term we went around to classes and had weekly meeting to introduce students to synthetic biology through a series of guest lectures. During the spring students participated in brainstorming projects related to the sponsoring Faculty advisors labs (Baker, Mougous, Klavins, Sauro) and came up with the project they wanted to carry out. Over the summer, students completed all of the experimental work, but worked closely with both graduate students and faculty advisors to plan the most pertinent experiments and make the most of our limited time.

The Gram Positive Therapeutic

Chris Eiben, Sean Wu, Peixian Liu, David Zong, Ivan Huang, Cindy Wu, Denny Luan designed, expressed, purified, and characterized CapD, CapD_CP, and the 87 CapD_CP variants.

The Gram Negative Therapeutic

Matthew Harger, Matthew Coyne, and Jesa Landis cloned and characterized the Tse2/Tsi2 locus into BioBrick parts.

Laura Austin and Justin De Leon recombineered and characterized the T7 promoter into the T6SS fosmid.

New Tools

Jeff Johnson developed the WikiDust and PartsRobot software. Jeff also wrote BBF RFC 68. Michal Galdzicki and Professor Herbert M. Sauro read and revised the document.

Tomas Huber BioBricked the f1 origin, lacI repressor, and lacI regulated T7 promoter, taking them from the protein expression vector pET29b+ from Novagen. He also constructed and characterized 4 protein-based expression cassette's made from new AND old BioBricks.

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