Team:Washington/Protocols/Ligation

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(General Ligation Protocol)
 
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1uL T4 ligase
1uL T4 ligase
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incubate at <s>37C</s> no.  **Note that NEB website recommends 16C - room temp for ligations, we do ours at RT and it works well.  1 hour.
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Incubate at 16C overnight, or room temperature for 30 minutes to 1 hour.

Latest revision as of 02:40, 16 September 2011


General Ligation Protocol

7uL insert

1uL vector

1uL T4 ligase buffer

1uL T4 ligase

Incubate at 16C overnight, or room temperature for 30 minutes to 1 hour.