Team:Washington/Protocols/Induc studies.

From 2011.igem.org

(Difference between revisions)
(Created page with "{{Template:Team:Washington/Templates/Top}} =Induction Studies of Gene-Protein Fusions (mamK-sfGFP and mamI-sfGFP)= # Obtain a tube and fill it with 5 mL TB + Amp + Chlor. # Ob...")
 
Line 10: Line 10:
# Prepare a microscope slide of each sample and Image using a microscope.
# Prepare a microscope slide of each sample and Image using a microscope.
#* Pipet ~ 5 ul of each sample onto separate slides.
#* Pipet ~ 5 ul of each sample onto separate slides.
-
#* Cover the samples with agarose while trying not to introduce any bubbles.
+
#* Carefully cover the samples with agarose containing M9 + Amp + Kan + IPTG

Latest revision as of 22:38, 15 September 2011


Induction Studies of Gene-Protein Fusions (mamK-sfGFP and mamI-sfGFP)

  1. Obtain a tube and fill it with 5 mL TB + Amp + Chlor.
  2. Obtan another tube and fill it with 1 mL TB + Amp + Chlor.
  3. Starting with an already prepared PBS broth, pipet 20 uL into each tube.
  4. Add 200 uM IPTG into each tube and incubate at room temperature.
  5. Prepare a microscope slide of each sample and Image using a microscope.
    • Pipet ~ 5 ul of each sample onto separate slides.
    • Carefully cover the samples with agarose containing M9 + Amp + Kan + IPTG