Team:UT Dallas/immunobot intro

From 2011.igem.org

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Stanford iGEM 2009 (<a href="https://2009.igem.org/Team:Stanford/" target='_blank'>https://2009.igem.org/Team:Stanford/</a>)
Stanford iGEM 2009 (<a href="https://2009.igem.org/Team:Stanford/" target='_blank'>https://2009.igem.org/Team:Stanford/</a>)
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<b>Specifications</b>
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<b>Sensing Module</b>
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Chimeric FGFR-ToxR ReceptorFibroblasts play a critical role in the proliferative stage of wound healing. Fibroblast growth
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Fibroblasts play a critical role in the proliferative stage of wound healing. Fibroblast growth
factors (FGFs) promote increased fibroblast cell differentiation and proliferation. Therefore
factors (FGFs) promote increased fibroblast cell differentiation and proliferation. Therefore
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FGFs can represent wound signals for our immunobot sensing module. FGFs work with heparan
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FGFs can represent wound signals for our immunobot sensing module. FGFs work with Heparin
sulfate-containing proteoglycans to induce the FGF receptor (FGFR). Once induced, FGFR
sulfate-containing proteoglycans to induce the FGF receptor (FGFR). Once induced, FGFR
dimerizes and triggers FGF signal transduction.
dimerizes and triggers FGF signal transduction.
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controlled chemotaxis, hence completing the linkage between wound signals and movement.
controlled chemotaxis, hence completing the linkage between wound signals and movement.
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<b>Chemotaxis</b>
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<b>Taxis Module</b>
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Bacterial chemotaxis in our chassis (E. coli DH5a) involves interactions of transmembrane
Bacterial chemotaxis in our chassis (E. coli DH5a) involves interactions of transmembrane
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autophosphorylation. At this point, CheY interacts with the activated phosphorylated CheA via
autophosphorylation. At this point, CheY interacts with the activated phosphorylated CheA via
diffusion and becomes phosphorylated itself by the newly activated CheA. CheY then forms a
diffusion and becomes phosphorylated itself by the newly activated CheA. CheY then forms a
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complex with the flagellar motor proteins (FLiM) and consequently induces clockwise rotations
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complex with the flagellar motor proteins (FLiM) and induces clockwise rotations
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of the flagella, which causes the cell to tumble in place with no net movement. Alternatively,
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of the flagella. This causes the cell to tumble in place with no net movement. Alternatively,
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CheZ, another chemotactic regulator protein, dephosphorylates CheY, which decreases the
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CheZ, another chemotactic regulator protein, dephosphorylates CheY. This decreases the
cellular concentration of CheY, so less phosphorylated CheY binds the FLiM protein. As a
cellular concentration of CheY, so less phosphorylated CheY binds the FLiM protein. As a
result, the flagella instead rotate counter clockwise, inducing straight line movement as the
result, the flagella instead rotate counter clockwise, inducing straight line movement as the

Latest revision as of 01:29, 29 September 2011