Team:UST-Beijing/Parts

From 2011.igem.org

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     <td><img src="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" alt="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" width="558" height="212"></td>
     <td><img src="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" alt="https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg" width="558" height="212"></td>
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     <td>PR, a abbreviation of proteorhodopsin,is a kind of membrane protein  
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     <td>PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part, K603000 designed by Danyang Wang, was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.</td>
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which was discovered in 2000 through shortgun sequencing of seawater off the coast of California. PR is a light-activated proton pump and generates  
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a pmf which is short for proton motive force. K603000
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  Designed by Danyang Wang   (Team: UST-Beijing). The part is constructed as below: We replace the PR's precusor sequence with   human cytochrome oxidase subunit 4 isoform 1, mitochondrial precursor.   Meanwhile, all the codons are redesigned according to codon usage bias for Homo   sspiens.</td>
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Latest revision as of 02:16, 13 September 2011

 

 
https://static.igem.org/mediawiki/2011/6/62/PSB1AC3.jpg PR, an abbreviation for proteorhodopsin, is a transmembrane protein which was discovered in 2000 through shortgun genome sequencing of unculturable bacteria isolated from the seawater off the coast of California. PR is a light-activated proton pump and generates a pmf which is short for proton motive force. This part, K603000 designed by Danyang Wang, was constructed as below: We replaced the PR's precusor sequence with the leader peptide of human cytochrome oxidase subunit 4 isoform 1, targeting it to mitochondrial inner membrane. Meanwhile, all the codons were re-designed according to codon usage bias for Homo sapiens.
   

 

 

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