Team:UNIST Korea

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<font size="12"><font color="blue">SELF LESS GMOs</font></font>
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Genetically Modified Organisms (GMOs) pose a significant threat to human health and the environment. Despite being a promising candidate in various processes such as production of drugs and bio-commodities, GMOs do not experience a warm welcome from the human society. Fear of destruction of the natural biota
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suppresses all of the benefits offered by GMOs. Horizontal gene transfer (HGT) from GMOs to some pathogenic microbes, which leads to superbugs, is yet another major concern with GMOs. Here, we design a GMO with a built-in triggered-suicide system that is inducible by environmental signals (such as light, oxygen, heavy metals or quorum sensing system). This selfless GMO would kill itself on receiving environmental cues when it is released from its native completely closed system (fermentor or laboratory) to the open system (surrounding environment). We propose the use of a novel suicide platform using the genes coding for the restriction enzyme, DpnI that would chew up all methylated DNAs. This novel suicide platform may help prevent HGT and in turn enhance the potential benefits offered by GMOs.''<br /></font>
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-
top: 0;
+
-
left: 0;
+
-
width: 100%;
+
-
height: 100%;
+
-
background: transparent;
+
-
z-index: 1101;
+
-
}
+
-
 
+
-
#fancybox-close {
+
-
position: absolute;
+
-
top: -15px;
+
-
right: -15px;
+
-
width: 30px;
+
-
height: 30px;
+
-
background: transparent url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png') -40px 0px;
+
-
cursor: pointer;
+
-
z-index: 1103;
+
-
display: none;
+
-
}
+
-
 
+
-
#fancybox-error {
+
-
color: #444;
+
-
font: normal 12px/20px Arial;
+
-
padding: 14px;
+
-
margin: 0;
+
-
}
+
-
 
+
-
#fancybox-img {
+
-
width: 100%;
+
-
height: 100%;
+
-
padding: 0;
+
-
margin: 0;
+
-
border: none;
+
-
outline: none;
+
-
line-height: 0;
+
-
vertical-align: top;
+
-
}
+
-
 
+
-
#fancybox-frame {
+
-
width: 100%;
+
-
height: 100%;
+
-
border: none;
+
-
display: block;
+
-
}
+
-
 
+
-
#fancybox-left, #fancybox-right {
+
-
position: absolute;
+
-
bottom: 0px;
+
-
height: 100%;
+
-
width: 35%;
+
-
cursor: pointer;
+
-
outline: none;
+
-
background: transparent url('https://static.igem.org/mediawiki/2011/c/c0/Blank.gif');
+
-
z-index: 1102;
+
-
display: none;
+
-
}
+
-
 
+
-
#fancybox-left {
+
-
left: 0px;
+
-
}
+
-
 
+
-
#fancybox-right {
+
-
right: 0px;
+
-
}
+
-
 
+
-
#fancybox-left-ico, #fancybox-right-ico {
+
-
position: absolute;
+
-
top: 50%;
+
-
left: -9999px;
+
-
width: 30px;
+
-
height: 30px;
+
-
margin-top: -15px;
+
-
cursor: pointer;
+
-
z-index: 1102;
+
-
display: block;
+
-
}
+
-
 
+
-
#fancybox-left-ico {
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -30px;
+
-
}
+
-
 
+
-
#fancybox-right-ico {
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -60px;
+
-
}
+
-
 
+
-
#fancybox-left:hover, #fancybox-right:hover {
+
-
visibility: visible; /* IE6 */
+
-
}
+
-
 
+
-
#fancybox-left:hover span {
+
-
left: 20px;
+
-
}
+
-
 
+
-
#fancybox-right:hover span {
+
-
left: auto;
+
-
right: 20px;
+
-
}
+
-
 
+
-
.fancybox-bg {
+
-
position: absolute;
+
-
padding: 0;
+
-
margin: 0;
+
-
border: 0;
+
-
width: 20px;
+
-
height: 20px;
+
-
z-index: 1001;
+
-
}
+
-
 
+
-
#fancybox-bg-n {
+
-
top: -20px;
+
-
left: 0;
+
-
width: 100%;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/9/92/Kaist2011_fancybox-x.png');
+
-
}
+
-
 
+
-
#fancybox-bg-ne {
+
-
top: -20px;
+
-
right: -20px;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -162px;
+
-
}
+
-
 
+
-
#fancybox-bg-e {
+
-
top: 0;
+
-
right: -20px;
+
-
height: 100%;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/4/42/Kaist2011_fancybox-y.png');
+
-
background-position: -20px 0px;
+
-
}
+
-
 
+
-
#fancybox-bg-se {
+
-
bottom: -20px;
+
-
right: -20px;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -182px;
+
-
}
+
-
 
+
-
#fancybox-bg-s {
+
-
bottom: -20px;
+
-
left: 0;
+
-
width: 100%;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/9/92/Kaist2011_fancybox-x.png');
+
-
background-position: 0px -20px;
+
-
}
+
-
 
+
-
#fancybox-bg-sw {
+
-
bottom: -20px;
+
-
left: -20px;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -142px;
+
-
}
+
-
 
+
-
#fancybox-bg-w {
+
-
top: 0;
+
-
left: -20px;
+
-
height: 100%;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/4/42/Kaist2011_fancybox-y.png');
+
-
}
+
-
 
+
-
#fancybox-bg-nw {
+
-
top: -20px;
+
-
left: -20px;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png');
+
-
background-position: -40px -122px;
+
-
}
+
-
 
+
-
#fancybox-title {
+
-
font-family: Helvetica;
+
-
font-size: 12px;
+
-
z-index: 1102;
+
-
}
+
-
 
+
-
.fancybox-title-inside {
+
-
padding-bottom: 10px;
+
-
text-align: center;
+
-
color: #333;
+
-
background: #fff;
+
-
position: relative;
+
-
}
+
-
 
+
-
.fancybox-title-outside {
+
-
padding-top: 10px;
+
-
color: #fff;
+
-
}
+
-
 
+
-
.fancybox-title-over {
+
-
position: absolute;
+
-
bottom: 0;
+
-
left: 0;
+
-
color: #FFF;
+
-
text-align: left;
+
-
}
+
-
 
+
-
#fancybox-title-over {
+
-
padding: 10px;
+
-
background-image: url('https://static.igem.org/mediawiki/2011/3/3f/Kaist2011_fancy_title_over.png');
+
-
display: block;
+
-
}
+
-
 
+
-
.fancybox-title-float {
+
-
position: absolute;
+
-
left: 0;
+
-
bottom: -20px;
+
-
height: 32px;
+
-
}
+
-
 
+
-
#fancybox-title-float-wrap {
+
-
border: none;
+
-
border-collapse: collapse;
+
-
width: auto;
+
-
}
+
-
 
+
-
#fancybox-title-float-wrap td {
+
-
border: none;
+
-
white-space: nowrap;
+
-
}
+
-
 
+
-
#fancybox-title-float-left {
+
-
padding: 0 0 0 15px;
+
-
background: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png') -40px -90px no-repeat;
+
-
}
+
-
 
+
-
#fancybox-title-float-main {
+
-
color: #FFF;
+
-
line-height: 29px;
+
-
font-weight: bold;
+
-
padding: 0 0 3px 0;
+
-
background: url('https://static.igem.org/mediawiki/2011/9/92/Kaist2011_fancybox-x.png') 0px -40px;
+
-
}
+
-
 
+
-
#fancybox-title-float-right {
+
-
padding: 0 0 0 15px;
+
-
background: url('https://static.igem.org/mediawiki/2011/d/d5/Kaist2011_fancybox.png') -55px -90px no-repeat;
+
-
}
+
-
 
+
-
/* IE6 */
+
-
 
+
-
.fancybox-ie6 #fancybox-close { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/c/cb/Kaist2011_fancy_close.png', sizingMethod='scale'); }
+
-
 
+
-
.fancybox-ie6 #fancybox-left-ico { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/4/40/Kaist2011_fancy_nav_left.png', sizingMethod='scale'); }
+
-
.fancybox-ie6 #fancybox-right-ico { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/2/2b/Kaist2011_fancy_nav_right.png', sizingMethod='scale'); }
+
-
 
+
-
.fancybox-ie6 #fancybox-title-over { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/3/3f/Kaist2011_fancy_title_over.png', sizingMethod='scale'); zoom: 1; }
+
-
.fancybox-ie6 #fancybox-title-float-left { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/f/f9/Kaist2011_fancy_title_left.png', sizingMethod='scale'); }
+
-
.fancybox-ie6 #fancybox-title-float-main { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/c/c7/Kaist2011_fancy_title_main.png', sizingMethod='scale'); }
+
-
.fancybox-ie6 #fancybox-title-float-right { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/a/ae/Kaist2011_fancy_title_right.png', sizingMethod='scale'); }
+
-
 
+
-
.fancybox-ie6 #fancybox-bg-w, .fancybox-ie6 #fancybox-bg-e, .fancybox-ie6 #fancybox-left, .fancybox-ie6 #fancybox-right, #fancybox-hide-sel-frame {
+
-
height: expression(this.parentNode.clientHeight + "px");
+
-
}
+
-
 
+
-
#fancybox-loading.fancybox-ie6 {
+
-
position: absolute; margin-top: 0;
+
-
top: expression( (-20 + (document.documentElement.clientHeight ? document.documentElement.clientHeight/2 : document.body.clientHeight/2 ) + ( ignoreMe = document.documentElement.scrollTop ? document.documentElement.scrollTop : document.body.scrollTop )) + 'px');
+
-
}
+
-
 
+
-
#fancybox-loading.fancybox-ie6 div { background: transparent; filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/4/40/Kaist2011_fancy_loading.png', sizingMethod='scale'); }
+
-
 
+
-
/* IE6, IE7, IE8 */
+
-
 
+
-
.fancybox-ie .fancybox-bg { background: transparent !important; }
+
-
 
+
-
.fancybox-ie #fancybox-bg-n { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/e/ee/Kaist2011_fancy_shadow_n.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-ne { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/d/de/Kaist2011_fancy_shadow_ne.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-e { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/c/ce/Kaist2011_fancy_shadow_e.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-se { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/4/42/Kaist2011_fancy_shadow_se.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-s { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/7/76/Kaist2011_fancy_shadow_s.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-sw { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/a/a1/Kaist2011_fancy_shadow_sw.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-w { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/a/a9/Kaist2011_fancy_shadow_w.png', sizingMethod='scale'); }
+
-
.fancybox-ie #fancybox-bg-nw { filter: progid:DXImageTransform.Microsoft.AlphaImageLoader(src='https://static.igem.org/mediawiki/2011/7/70/Kaist2011_fancy_shadow_nw.png', sizingMethod='scale'); }
+
-
 
+
-
/* */
+
-
 
+
-
#content {border:none; position:relative; top:-20px; background-color:transparent;}
+
-
 
+
-
#globalWrapper {
+
-
clear:both;
+
-
width:100%;
+
-
position:absolute;
+
-
top:0px;
+
-
}
+
-
 
+
-
#contentSub {display:none;}
+
-
#siteSub {display:none;}
+
-
#search-controls {
+
-
width:300px;
+
-
 
+
-
        top:25px;
+
-
        right:25px;
+
-
        z-index:100;
+
-
}
+
-
 
+
-
input.searchButton {
+
-
position:absolute;
+
-
font-size:10pt;
+
-
font-family:"Arial";
+
-
font-weight:normal;
+
-
color:#FFFFFF;
+
-
}
+
-
 
+
-
#searchInput{
+
-
background-color:transparent;
+
-
z-index:100;
+
-
color:#FFFFFF;
+
-
font:Arial;
+
-
text-align:center;
+
-
 
+
-
background:url('https://static.igem.org/mediawiki/2011/2/2e/Kaist2011_head_searchbar.png') no-repeat;
+
-
background-position:0px 0px;
+
-
vertical-align:middle;
+
-
 
+
-
width: 184px;
+
-
height: 22px;
+
-
border: none;
+
-
 
+
-
}
+
-
 
+
-
#searchGoButton {
+
-
font-size:0pt;
+
-
background:url('https://static.igem.org/mediawiki/2011/5/5f/Kaist2011_head_arrow.png') no-repeat;
+
-
background-position:left 0px;
+
-
cursor:pointer;
+
-
cursor: hand; /* for IE 5.x */
+
-
 
+
-
width: 29px;
+
-
height: 29px;
+
-
border: none;
+
-
}
+
-
#mw-searchButton {
+
-
position:relative;
+
-
left:35px;
+
-
font-size:0pt;
+
-
background:url('https://static.igem.org/mediawiki/2011/a/a1/Kaist2011_head_search.png') no-repeat;
+
-
background-position:left 0px;
+
-
cursor:pointer;
+
-
cursor: hand; /* for IE 5.x */
+
-
 
+
-
width: 29px;
+
-
height: 29px;
+
-
border: none;
+
-
}
+
-
.firstHeading {display:none;}
+
-
 
+
-
#top-section {
+
-
height:80px; width:1000px;
+
-
border:none;
+
-
z-index:0;
+
-
}
+
-
 
+
-
<!--Remove the banner-->
+
-
#p-logo {position:relative; top:0px; z-index:0; height:1px; width:10px; overflow:hidden;}
+
-
#p-logo a{color:black;}
+
-
img[src*="/wiki/skins/common/images/wiki.png"]{
+
-
    visibility:hidden;
+
-
    top:-100px;
+
-
    width:10px;
+
-
    height:0px;
+
-
margin-top:20px;
+
-
    background:transparent;
+
-
}
+
-
 
+
-
#menubar{
+
-
clear:both;
+
-
top:0px;
+
-
z-index:1;
+
-
font-size:8pt;
+
-
font-family:"Arial";}
+
-
#menubar:hover{
+
-
background-color:transparent;
+
-
}
+
-
 
+
-
.left-menu {margin-top:0px; left:-20px;}
+
-
.left-menu ul {border:none;}
+
-
.right-menu {margin-top:0px; right:-5px;}
+
-
.right-menu ul {border:none;}
+
-
.right-menu ul a {background-color:transparent;}
+
-
.left-menu li a {color: #ffffff;}
+
-
.left-menu li a:visited {color: #ffffff;}
+
-
.left-menu li a:hover { color:#bbe0e9;}
+
-
.left-menu li a:active{ color:#bbe0e9; font-weight:bold;}
+
-
.right-menu li a {color: #000000;}
+
-
.right-menu li a:visited {color: #000000;}
+
-
.right-menu li a:hover {color:#ababab; text-decoration:none;}
+
-
.right-menu li a:active {color:#ababab; text-decoration:none;font-weight:bold;}
+
-
 
+
-
#menubar li {
+
-
        margin: 6px;
+
-
}
+
-
 
+
-
#catlinks {
+
-
position:relative;
+
-
top:5px;
+
-
left:-5px;
+
-
width:990px;
+
-
border:none;
+
-
background-color:transparent;
+
-
padding-top:5px;
+
-
clear: both;
+
-
}
+
-
 
+
-
#footer-box {width:965px; background-color:transparent; border:none; position:relative; top:-50px; padding-top:25px;}
+
-
#footer {
+
-
position:relative;
+
-
bottom:5%;
+
-
background-color:transparent;
+
-
font-size:8pt;
+
-
font-family:"Arial";
+
-
z-index:1;
+
-
}
+
-
#footer li a {color:#000000;}
+
-
#footer img {
+
-
margin:10px;
+
-
border:none;
+
-
}
+
-
#footer li a:visited {color::#000000;}
+
-
#footer li a:hover { color:#ababab; text-decoration:none; }
+
-
#footer li a:active{ color:#ababab; text-decoration:none; font-weight:bold}
+
-
body {
+
-
width=100%;
+
-
 
+
-
background-image:url('https://static.igem.org/mediawiki/2011/8/84/Kaist2011_menu_background.jpg');
+
-
background-repeat:no-repeat;
+
-
background-position:center top;
+
-
background-attachment: scroll;
+
-
 
+
-
background-color:#bbe0e9;
+
-
z-index:-100;
+
-
}
+
-
 
+
-
#kaistcontent{
+
-
background-color:white; -moz-opacity:1.0; filter:alpha(opacity=100); opacity:1.0;
+
-
width:815px;
+
-
font-family:Helvetica, Arial, Sans-serif;
+
-
font-size:10pt;
+
-
line-height:90%;
+
-
text-align:justify;
+
-
padding: 25px 75px 25px 75px;
+
-
}
+
-
 
+
-
#kaistcontent h2{
+
-
color:#215968;
+
-
font:Arial 13pt;
+
-
line-height:100%;
+
-
}
+
-
 
+
-
#kaistcontent h3{
+
-
color:#215968;
+
-
font-weight:normal;
+
-
font:Arial 11pt;
+
-
line-height:110%;
+
-
}
+
-
+
-
.upbg {
+
-
position: relative;
+
-
overflow:hidden;
+
-
top: -55px;
+
-
left: -145px;
+
-
width:0px;
+
-
height:0px;
+
-
z-index:-100;
+
-
}
+
-
</style>
+
-
 
+
-
</head>
+
-
 
+
<body>
<body>
-
<div>
+
<blockquote>
-
<a href="https://2011.igem.org/Team:KAIST-Korea/jihan"><img id="tag1" src="https://static.igem.org/mediawiki/2011/8/8f/Kaist2011_text_home.png" />
+
<background-image: url("https://2011.igem.org/File:Picture1.png ");>
-
</a>
+
<background-position: 50% 50%;>
-
</div>
+
<background-repeat: no-repeat;>
-
<!--contents here in-->
+
</body>
</body>
-
 
+
</HTML>
-
 
+
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<h2> <span class="mw-headline" id="Project_Description">Project Description</span></h2>
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E. Casso is an E. coli system engineered into art. This system consists of two types of genetically modified E. coli: the random signal generating E. coli (the “Brush E. coli”) and the color generating E. coli (the “Paint E. coli”).
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The Brush E. coli possesses genes (lasI, luxI, rhlI, and cinI) that produce quorum in its chromosomal DNA. Each Brush E. coli will secrete quorum molecules randomly associated with one of four colors among red, cyan, yellow, and green by a modified Cre-loxP mechanism.
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The Paint E.coli possesses plasmid vectors that express one of four colors of fluorescent proteins in response to the type of quorum it receives from the Brush E.coli. The quorum sensing complex completed in the Brush will bind to the ribosome binding site (RBS) of the corresponding fluorescent gene.
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Using this system, we can create art on an LP plate with the cells' “random” contribution.
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<iframe src="http://www.facebook.com/plugins/like.php?app_id=115152055242776&amp;href=http%3A%2F%2F2011.igem.org%2FTeam%3AKAIST-Korea&amp;send=false&amp;layout=standard&amp;width=200&amp;show_faces=false&amp;action=like&amp;colorscheme=bright&amp;font=arial&amp;height=35" scrolling="no" frameborder="0" style="text-align:justify;border:none; overflow:hidden; width:250px; height:40px; position:relative; left:10px;" allowTransparency="true"></iframe>
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Revision as of 10:48, 10 September 2011

Home Team Project Notebook Safety




SELF LESS GMOs





Genetically Modified Organisms (GMOs) pose a significant threat to human health and the environment. Despite being a promising candidate in various processes such as production of drugs and bio-commodities, GMOs do not experience a warm welcome from the human society. Fear of destruction of the natural biota suppresses all of the benefits offered by GMOs. Horizontal gene transfer (HGT) from GMOs to some pathogenic microbes, which leads to superbugs, is yet another major concern with GMOs. Here, we design a GMO with a built-in triggered-suicide system that is inducible by environmental signals (such as light, oxygen, heavy metals or quorum sensing system). This selfless GMO would kill itself on receiving environmental cues when it is released from its native completely closed system (fermentor or laboratory) to the open system (surrounding environment). We propose the use of a novel suicide platform using the genes coding for the restriction enzyme, DpnI that would chew up all methylated DNAs. This novel suicide platform may help prevent HGT and in turn enhance the potential benefits offered by GMOs.''

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