Team:UNIPV-Pavia

From 2011.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 6: Line 6:
<td>
<td>
<div style='text-align:center'><div class="thumbinner" style="width:100%;">
<div style='text-align:center'><div class="thumbinner" style="width:100%;">
-
<img alt="" src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage"  width="100%"></a></div></div>
+
<img alt="" src="https://static.igem.org/mediawiki/2011/b/b1/UNIPV_Pavia_Work_in_progress_scribblings.jpg" class="thumbimage"  width="93%"></a></div></div>
</td>
</td>
</tr>
</tr>
Line 39: Line 39:
-
<div style="width:700px;margin:10px auto;">
+
<div style="width:550px;margin:10px auto;">
<div class="slider-wrapper theme-default">
<div class="slider-wrapper theme-default">
<div class="ribbon"></div>
<div class="ribbon"></div>
-
<div id="nivoslider-125" class="nivoSlider" style="width:700px;height:470px;">
+
<div id="nivoslider-125" class="nivoSlider" style="width:550px;height:366px;">
-
 
+
-
<img src="https://static.igem.org/mediawiki/2011/8/81/UNIPVteam1.jpg" title="UNIPV iGEM team" alt="" />
+
-
<img src="https://static.igem.org/mediawiki/2011/1/13/UNIPV_team3.jpg" title="UNIPV iGEM team" alt="" />
+
-
<img src="https://static.igem.org/mediawiki/2011/d/d3/UNIPV_Team4.jpg" title="UNIPV iGEM team" alt="" />
+
 +
<img src="https://static.igem.org/mediawiki/2011/2/2d/UNIPV_Hometeam1.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/0/0a/UNIPV_Hometeam2.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/e/e1/UNIPV_Hometeam3.jpg" title="UNIPV iGEM team" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f2/UNIPV_Hometeam4.jpg" title="Work in progress" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/9/9b/UNIPV_Home1.jpg" title="Pavia and the river Ticino" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/a/a2/UNIPV_Home2.jpg" title="University of Pavia" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f7/UNIPV_Home3.jpg" title="Faculty of Engineering" alt="" />
 +
<img src="https://static.igem.org/mediawiki/2011/f/f0/UNIPV_Home4.jpg" title="Scientific campus" alt="" />
</div>
</div>

Latest revision as of 13:14, 19 September 2011

UNIPV TEAM 2011


The project

Our work aims at implementing the engineering concept of closed-loop control in E. coli, exploiting quorum sensing. As a proof of concept, we designed a simple genetic controller that regulates the concentration of 3OC6-HSL signalling molecule around a user-defined set-point. The controlled variable (3OC6-HSL) increases as a function of the exogenous anhydroTetracycline input, that triggers LuxI expression. The controller senses the 3OC6-HSL concentration and activates the production of AiiA, that degrades it. To observe the desired behaviour, a fine tuning of the system was necessary. The transcriptional/translational strength of the regulatory elements (promoter+RBS in several combinations) and the enzyme activities were measured and exploited to identify a mathematical model able to predict the behaviour of the controlled system. These predictions made possible an in silico rational fine tuning of the circuit: the most promising modules were selected and assembled into the final circuit, avoiding a cost and time expensive combinatorial approach.


You can add captions too…



Retrieved from "http://2011.igem.org/Team:UNIPV-Pavia"