Another feature of pINDEL is that its replication origin is not functional at 42°C due to the thermosensitivity of the RepA101Ts protein. This protein initiates replication at the ori site in permissive conditions (30°C) and is inactive at 42°C.
The deletion function and the thermosensitivity of the plasmid both could be shown to be working as intended (see functional parameters). The insertion function couldn't be properly tested