Team:UIUC-Illinois

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          <div class="title">eChive</div>
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      <div class="title"><center>E.chiver - Dynamically Efficient Bacteria:</center></div>
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           <div class="desc">Please read below for the project description.</div>
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           <div class="desc"><center>Creating a "Bacterial Filing Cabinet"</center></div>
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       <div class="title"><center>University of Illinois - International Genetically Engineered Machine</center></div>
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         <div class="title">What is eChiver?</div>
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        <div class="desc"><img src="https://static.igem.org/mediawiki/2011/e/e5/Uiuc_Echiver.jpg" width="112" height="162" align="left" />Our project, E. chiver, drew inspiration from the commonly used CRIM system, a series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes. Our E. chiver system adds several elements yielding new applications. Our team designed two E. chiver constructs utilizing Lambda and P21 machinery. Each can in theory be used to shuttle a plasmid construct between two forms: a single chromosomal insert and a high copy number plasmid. In their current designs the systems must function separately, but possible routes have been identified by our team to make the co-functioning of these systems possible. We can see elements of our project being used in drug delivery systems as a method to keep a gene of interest dormant unless in the correct condition/location, and with further exploration into the co-functioning routes it may be used to create a ‘bacterial filing cabinet’.</div>
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          <div class="black-box-text">June 20th, 2011<br />Quick Note about what has been going on in lab recently. I'm not sure what to write here for now so I'm going to blan some more until this space filles up. Blab Blab Blab Blab Blab Blab</div>
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          <div class="black-box-text">June 20th, 2011<br />Quick Note about what has been going on in lab recently. I'm not sure what to write here for now so I'm going to blan some more until this space filles up. Blab Blab Blab Blab Blab Blab</div>
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          <div class="black-box-text">June 20th, 2011<br />Quick Note about what has been going on in lab recently. I'm not sure what to write here for now so I'm going to blan some more until this space filles up. Blab Blab Blab Blab Blab Blab</div>
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        <div class="core-content-block-t2-left-title">Dynamic</div>
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         <div class="desc">Responding to stimuli on the fly with rapid changes in state and function.</div>
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          <div class="black-box-text">June 20th, 2011<br />Quick Note about what has been going on in lab recently. I'm not sure what to write here for now so I'm going to blan some more until this space filles up. Blab Blab Blab Blab Blab Blab</div>
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         <div class="title">About the eChive Project</div>
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         <div class="desc">Our project is being deemed the ‘bacterial filing cabinet’.  The idea is to introduce two constructs that express distinct phenotypes into the chromosome of E. coli.  The single copy nature of these insertions will prevent either phenotype from being easily detectable.  A phenotype will be pulled out of its dormant single copy state by excision of the construct and replication into a high copy number vector.  Phenotypes can by switched by reinserting the first construct, destroying any remaining plasmid-form construct, then excising the second construct and replicating it to a high copy number.  The excision and integration of each construct will be under the control of a lambdoid phage system.  </div>
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         <div class="core-content-block-t2-left-title">Efficient</div>
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        <div class="desc">While maximizing efficiency in both the active and passive states.</div>
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         <div class="title">Recent Photos From The Lab</div>
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         <div class="core-content-block-t2-left-title">Multi-purpose</div>
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        <div class="desc">A variety of possible stimuli that each induce a different response.</div>
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          <div class="name">Amanda Chang</div>
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          <div id="sponsors-usscien"><a href="http://www.usascientific.com/"><img src="https://static.igem.org/mediawiki/2011/8/8f/Uiuc2011_sponsor_usa_scientific.jpg" alt="USA Scientific" /></a></div>
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         <div id="sponsors-abe"><a href="http://abe.illinois.edu/"><img src="https://static.igem.org/mediawiki/2011/8/84/Uiuc2011_sponsor_abe.jpg" alt="Department of Agricultural and Biological Engineering" /></a></div>
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          <div id="quote-author">-Will</div>
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<center><a href="https://www.vwrsp.com/"><font color="white">VWR</font></a> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; <a href="http://www.fishersci.com/"><font color="white">Fisher Scientific</font></a> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  <a href="http://www.labcon.com/"><font color="white"> labcon </font></a></center>
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Latest revision as of 02:02, 14 October 2011

University of Illinois iGEM Team
Echiver_logo
E.chiver - Dynamically Efficient Bacteria:
Creating a "Bacterial Filing Cabinet"
University of Illinois - International Genetically Engineered Machine
What is eChiver?
Our project, E. chiver, drew inspiration from the commonly used CRIM system, a series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes. Our E. chiver system adds several elements yielding new applications. Our team designed two E. chiver constructs utilizing Lambda and P21 machinery. Each can in theory be used to shuttle a plasmid construct between two forms: a single chromosomal insert and a high copy number plasmid. In their current designs the systems must function separately, but possible routes have been identified by our team to make the co-functioning of these systems possible. We can see elements of our project being used in drug delivery systems as a method to keep a gene of interest dormant unless in the correct condition/location, and with further exploration into the co-functioning routes it may be used to create a ‘bacterial filing cabinet’.
Who We Are
Amanda Chang
"A watched gel never runs"
Dynamic
Responding to stimuli on the fly with rapid changes in state and function.
Efficient
While maximizing efficiency in both the active and passive states.
Multi-purpose
A variety of possible stimuli that each induce a different response.
Sponsors
Micro and Nanotechnology Laboratory
Integrated DNA Technologies
GenScript
USA Scientific
Department of Agricultural and Biological Engineering
The Institute for Genomic Biology
VWR           Fisher Scientific           labcon

Retrieved from "http://2011.igem.org/Team:UIUC-Illinois"