Team:UIUC-Illinois

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Revision as of 03:24, 29 September 2011

University of Illinois iGEM Team
Echiver_logo
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E.chiver - Dynamically Efficient Bacteria:
Creating a "Bacterial Filing Cabinet"
University of Illinois - International Genetically Engineered Machine
What is eChiver?
Our project, E. chiver, drew inspiration from the commonly used CRIM system, a series of plasmids that allows the user to integrate constructs into lambdoid phage sites common to many bacterial chromosomes. Our E. chiver system adds several elements yielding new applications. Our team designed two E. chiver constructs utilizing Lambda and P21 machinery. Each can in theory be used to shuttle a plasmid construct between two forms: a single chromosomal insert and a high copy number plasmid. In their current designs the systems must function separately, but possible routes have been identified by our team to make the co-functioning of these systems possible. We can see elements of our project being used in drug delivery systems as a method to keep a gene of interest dormant unless in the correct condition/location, and with further exploration into the co-functioning routes it may be used to create a ‘bacterial filing cabinet’.
Who We Are
Amanda Chang
"A watched gel never runs"
Dynamic
Responding to stimuli on the fly with rapid changes in state and function.
Efficient
While maximizing efficiency in both the active and passive states.
Multi-purpose
A variety of possible stimuli that each induce a different response.
Sponsors
Micro and Nanotechnology Laboratory
Integrated DNA Technologies
GenScript
USA Scientific
Department of Agricultural and Biological Engineering
The Institute for Genomic Biology

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