Team:UEA-JIC Norwich/Weekfive

From 2011.igem.org

University of East Anglia-JIC

UNIVERSITY OF EAST ANGLIA-JOHN INNES CENTRE


Mon5.jpg

A start to the week with everyone contemplating what has already been done and what we think is best to move forward successfully with the project, unbelievable how fast week 5 came along. We also performed a transformation in E.coli using the G-Luc sent to from cambridge.

Tue5.jpg

Our E.coli cells with the G-Luc plasmid grew nicely and we then picked off a colony to make a culture which was left to grow overnight.

Wed5.jpg

Today a miniprep was performed on our transformed E.coli cells containing the G-Luc plasmid, it looks like everything went according to plan. A glycerol stock of the transformed cells was also made so we can use them for future experiments.

Thur5.jpg

The Moss people of the team decided to start pouring plates today for a near future transformation, unfortunately right after forty plates had been poured one of the members of the group came to a horrific realisation that they had missed including an essential ingredient. All plates had to be discarded, what a nightmare. A gel of our miniprep from the previous day was performed today, sadly there was no presence of a band for the G-Luc. Today the team did not have much luck.

Fri5.jpg

Another attempt miniprepping the transformed E.coli cells with G-Luc was performed today, all hoping tomorrow we see a band present in the gel.

Kimberley met with Kay Yeoman to finalise her lesson plan for the first outreach event.