Team:UEA-JIC Norwich/Notebook

From 2011.igem.org

Revision as of 15:18, 23 June 2011 by Benjevans1 (Talk | contribs)


Lab Journal

Monday 20th June - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!

Tuesday 21st June- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.

Wednesday 22nd June- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.