From 2011.igem.org
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| - | <p><strong>Monday 20th June</strong> - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!</P> | + | <p>We began our Laboratory work on Monday the 20th of June. Click on a date below to see the wet work we did that day. If indicated, select the 'Methods' tab to view the protocol we followed to conduct our research. Enjoy!</p> |
| - | <p><strong>Tuesday 21st June</strong>- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.</p>
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| - | <p><strong>Wednesday 22nd June</strong>- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.</P>
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| - | <p><strong>Thursday 23rd June</strong> - Made glycerol stock solutions of all the successfully transformed E. coli cells from yesterday so that we will have an unlimited supply for the rest of the iGEM project.</p>
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Revision as of 15:36, 23 June 2011
Lab Journal
We began our Laboratory work on Monday the 20th of June. Click on a date below to see the wet work we did that day. If indicated, select the 'Methods' tab to view the protocol we followed to conduct our research. Enjoy!
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