Team:UC Davis/Notebook

From 2011.igem.org

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== Week 6 ==
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<h3>Week Selection</h3>
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--Monday --
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We weren't able to get all the parts for Gibson-ing our construct together.  We're still waiting on the 3 repressor PCRs and the pBAD promoter part.  The other's have been extracted and have decent concentrations for a Gibson mix.
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_1">1</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_2">2</a></td>
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Today we transformed pBAD+AraC for PCRing tomorrow.   
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_3">3</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_4">4</a></td>
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We also proceeded with the construction of our testing plasmid. We digested C0040, C0012, C0051 at XbaI and PstI so that we could ligate it into B0034, which we cut at SpeI and PstI. Additionally, we cut all of our promoter-GFP parts at EcoRI and SpeI, so that we could ligate them with I13453, cut at EcoRI and XbaI. We ran them on a gel, purified them, and plan on ligating and transforming them tomorrow.
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_5">5</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_6">6</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_7">7</a></td>
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  <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_8">8</a></td>
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  <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_9">9</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_10">10</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_11">11</a></td>
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  <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_12">12</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_13">13</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_14">14</a></td>
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  <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_15">15</a></td>
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<td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_16">16</a></td>
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<h1>Notebook</h1>
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Welcome to our notebook. Here, you may will find summaries of our progress, pictures from throughout the summer, and information on the protocols we use in our lab.<br><br>
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You may select a week above to travel directly to it, or begin at Week 1 <a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_1"><font style="color:#EE3333; size:40px;"> here</font></a>.
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If you're interested in what we've been up to since the first jamboree, you can see our progress <a href="https://2011.igem.org/Team:UC_Davis/Notebook/Post_American_Jamboree"><font style="color:#EE3333; size:40px;">here</font><a>.
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We ligated and transformed yesterday's gel purifications today. if this works, we'll be very close to finishing construction!
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<h2>Gallery</h2>
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We have a ton of photos in our gallery that didn't end up being used elsewhere on the website, including a few that were too silly to post anywhere else. Check them out <a href="https://2011.igem.org/Team:UC_Davis/Gallery"><font style="color:#EE3333; size:40px;"> here</font></a>.<br><br>
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Strange! Our plates from yesterday are covered with lots of green colonies, even the parts that don't contain GFP! We're going to do some tests; perhaps our media is contaminated, or the water that was used for ligations. We will re-ligate these parts, and re-digest from the minipreps. We ran these re-digestions on gel in order to extract the segment we wanted, but noticed some odd things about the bands. In the lanes containing our vectors, there were three bands where we would expect to only see one. This indicated to us that there was likely some contamination before our digestion. We went ahead and transformed the re-ligated gel purifications from earlier this week.
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<h2>Protocols</h2>
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Looking for the mix for our mutagenic PCR protocol? Performing a ligation and you're not sure how much vector to use? Check out our <a href="https://2011.igem.org/Team:UC_Davis/Protocols"><font style="color:#EE3333; size:40px;"> Protocols</font></a> page for step-by-step instructions on how to replicate our experiments.<br>
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Took a picture of Nick. He wasn't too happy. We fixed it.
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--Thursday--
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Once again, our plates are contaminated with green colonies. After some deliberation, we believe that our old media was contaminated with GFP-containing bacteria, and that media was used to culture some of our parts prior to a miniprep. Those minipreps, then, have both plasmids. We're going to re-streak the parts from glycerol stocks, and make new minipreps.
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Aaron made the bench into an igloo today complete with dry ice effects.  
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We also re-transformed C0051+E0240. Let's hope it works this time.
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== Week 7 ==
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--Monday 7/25/11--
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We continued with construction today and, once again, extracted the cut Promoters+GFP and repressors from a gel. We ligated the promoters+GFP in from of I13453 and the repressors before B0034. Hopefully this is the last time we will have to ligate and transform these parts.
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--Tuesday 7/26/11--
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The insert controls for yesterday's transformations looked good, but there were still a good amount of colonies on the vector controls. We decided to do a PCR screen of these parts to make sure that they were the right length.
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Today was also "Tip Day"! We filled enough tip boxes to last us a quite a while.
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== Week 8 ==
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== Week 9 ==
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== Week 10 ==
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Latest revision as of 21:05, 28 October 2011

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Criteria

View our judging criteria for iGEM 2011 here.

Week Selection

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Notebook

Welcome to our notebook. Here, you may will find summaries of our progress, pictures from throughout the summer, and information on the protocols we use in our lab.

You may select a week above to travel directly to it, or begin at Week 1 here.

If you're interested in what we've been up to since the first jamboree, you can see our progress here.

Gallery

We have a ton of photos in our gallery that didn't end up being used elsewhere on the website, including a few that were too silly to post anywhere else. Check them out here.

Protocols

Looking for the mix for our mutagenic PCR protocol? Performing a ligation and you're not sure how much vector to use? Check out our Protocols page for step-by-step instructions on how to replicate our experiments.

Retrieved from "http://2011.igem.org/Team:UC_Davis/Notebook"