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Team:Tsinghua/Notebook/1 September 2011
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
[[OmpA-sh3 parts]] | [[OmpA-sh3 parts]] | ||
| - | draw line | + | draw line |
| - | pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate | + | pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate |
| - | --[[User:Zora|Zora]] 02: | + | --[[User:Zora|Zora]] 02:52, 2 September 2011 (CDT) |
[[new plasmid]] | [[new plasmid]] | ||
Revision as of 07:52, 2 September 2011
draw line
pick 2 tubes of sequenced OmpA-Sh3 culture. Draw line on Chl+ plate --Zora 02:52, 2 September 2011 (CDT)
2.digest
pSB1C3 5ug digest with NEB EcoRI&SpeI
--Zora 01:28, 2 September 2011 (CDT)
3.gel analysis
OmpA-HIV cleavage site-Sh3 digested done by ZLY
pSB1C3 digested
--Zora 01:28, 2 September 2011 (CDT)
4.gel extraction
OHS vector Conc. 38ng/ul pSB1C3 vector Conc. 55ng/ul
--Zora 01:28, 2 September 2011 (CDT)
