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Team:Tokyo Tech/Projects/Urea-cooler/method
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| - | <h1> | + | <h1> Urea assay method </h1> |
| + | <h2 id="Overall1">1. Preparation for urea concentration assay</h2> | ||
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| + | <li>A single colony of cells transformed with mock, pTrc-rocF or pTrc-rocF-Arg box was inoculated into 3mL of LB with kanamycin and grown to saturation at 37℃.</li> | ||
| + | <li>The saturated culture was diluted 50-fold, grown till the log phase (OD600 = 0.5).</li> | ||
| + | <li>The culture was induced with 1mM IPTG at 37℃ for 1 hour. </li> | ||
| + | <li>4. 2mL of culture was centrifuged at 9,000 rmp for 1 minute and the supernatant fluid was used as a sample for urea concentration assay. </li> | ||
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Revision as of 10:52, 4 October 2011
Urea assay method
1. Preparation for urea concentration assay
- A single colony of cells transformed with mock, pTrc-rocF or pTrc-rocF-Arg box was inoculated into 3mL of LB with kanamycin and grown to saturation at 37℃.
- The saturated culture was diluted 50-fold, grown till the log phase (OD600 = 0.5).
- The culture was induced with 1mM IPTG at 37℃ for 1 hour.
- 4. 2mL of culture was centrifuged at 9,000 rmp for 1 minute and the supernatant fluid was used as a sample for urea concentration assay.
