Team:Tokyo Tech/Projects/Urea-cooler/data

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Urea-cooler Assay data

1. Characterization of rocF and Arg box

1.1 Materials

Expression plasmids used in this study are shown in Table 1.

TABLE 1. Expression plasmids used for Charcterization of rocF and Arg box
designation pSB3K3 pSB6A1
mock PlacIQ Alcohol-dehydrogenase(promoter-less)
rocF Ptrc-rocF Alcohol-dehydrogenase(promoter-less)
rocF+Arg box Ptrc-rocF Arg box
Strain MG1655 was transformed with either mock, tocF or rocF + Arg. As shown in Table 1, rocF gene was introduced on pSB3K3 and Arg boxes were introduced on pSB6A1.

1.2 Methods

Methods for this study are described here

1.3 Results

Each sample was assayed in duplicate urea concentration detected in each sample is shown in Table 2. Fig. 2 shows the average of these 2 values.

TABLE 2. Urea concentrations detected in duplicated designation pSB3K3 pSB6A1 mock PlacIQ Alcohol-dehydrogenase(promoter-less) rocF Ptrc-rocF Alcohol-dehydrogenase(promoter-less) rocF+Arg box Ptrc-rocF Arg box

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