Team:Rutgers

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<title>Rutgers 2011 iGEM Team: Complex Circuits in Synthetic Biology</title>
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     <td height="58" colspan="2" td background="https://static.igem.org/mediawiki/2011/9/96/Stripe.png"><p class="style3">RUTGERS iGEM TEAM WIKI<br />
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     <td width="9%" valign="top" class="stuff"><p><img src="http://gears.rutgers.edu/images/top_smallest.png" width="1000" height="278" class="imgshadow0" /></p></td>
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          <td colspan="3" td background="stripe.png"><h2> <span class="shadow">Welcome</span><br />
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          <td colspan="3" class="stuff"><p>For the Rutgers iGEM Team, complex is simple! The Rutgers iGEM Team has designed two complex genetic circuits, Etch-a-Sketch and Full Adder, and created a software tool, MYS!S. The Etch-a-Sketch circuit enables a lawn of bacteria to be drawn on with a laser. This seemingly inconsequential task presents many engineering challenges: the bacteria need to be sensitive in order to respond to a laser pulse, yet selective to use in ambient lighting. The second circuit allows bacteria to emulate a digital full adder. The circuit makes use of individually non-functional split reporters that can reform functional reporters with the help of fused “zipper” domains. In addition to the circuit, we have made easily fuse-able biobricks of these domains in order to facilitate the engineering of more split proteins, which should assist in the creation of logic circuits. MYS!S aims to improve the parts registry by checking and giving directions to modify Biobricks to conform to assembly standards.</p>
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            <p align="center"> <a href="https://2011.igem.org/Team:Rutgers/Team"><img src="http://www.rutgersigem.com/_/rsrc/1308671049435/team-members/group_pic_smaller.PNG"/></a></p></td>
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          <td width="25%" background="stripe.png" td><h2 class="shadow"> Etch-a-Sketch</h2></td>
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          <td width="25%" background="stripe.png" td><h2 class="shadow"> Full Adder</h2></td>
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          <td background="stripe.png" td><h2 class="shadow">  Mysis</h2></td>
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          <td width="33%" valign="top" span class="stuff"><p>The Etch-a-Sketch project aims to create a lawn of bacteria that can be drawn on with a laser pointer. This seemingly inconsequential task actually presents many interesting engineering challenges. In particular, the bacteria need to be extremely sensitive in order to respond to a short light pulse from a laser, but they still must be “selective” enough to use in ambient lighting.</p>
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            <p>We have designed a novel genetic switch that we hope will tackle these problems. If our work will serve as a useful model for future projects that require massive signal amplification. In particular, researchers creating biosensors may find our work very helpful.</p>
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            <p><a href="https://2011.igem.org/Team:Rutgers/EAS1"><img src="https://static.igem.org/mediawiki/2011/7/70/More.png" width="128" height="44" /></a></p>
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          <td width="33%" valign="top" span class="stuff"><p>The Full Adder project seeks to create bacteria that can mimic a digital full adder. This seemingly complex problem can be greatly simplified if we use a certain simple “encoding” on the outputs of the full adder. The full adder incorporates different types of AND gates, including one that incorporates split protein interactions with zipper linker domains. By allowing proteins to be split and reassociated, the zipper domains promise to bring new possibilities to future iGem projects.</p>
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            <p>Our insights may prove useful to any genetic engineer or synthetic biologist working on highly complex systems. The zipper linker domains create possibilities of new AND interactions, making complexity in future biologic circuits simple. The bacterial full adder may very well become the ancestor to more complicated biological calculators in the future.</p>
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            <p><a href="https://2011.igem.org/Team:Rutgers/FA1"><img src="https://static.igem.org/mediawiki/2011/7/70/More.png" width="128" height="44" /></a></p></td>
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          <td width="33%" valign="top" span class="stuff"><p>A major problem with the current Parts Registry, a library of BioBricks submitted by iGEM teams, is that many parts do not strictly conform to the BioBrick standard which makes certain operations extremely difficult. Rutger's iGEM software team strives to provide a tool to improve the standard parts registry by checking, and if need be modifying, the BioBrick parts.</p>
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            <p>The basic idea is that before a team submits their new BioBrick, it will run the genetic sequences through MYS!S. MYS!S will output the modified genetic sequence, BioCoder source code, and the lab protocol needed to change the unmodified sequence into the modified sequence The long term goal of the project is to further the automation of lab protocols by specifying them through algorithms.</p>
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            <p><a href="https://2011.igem.org/Team:Rutgers/MYS!S"><img src="https://static.igem.org/mediawiki/2011/7/70/More.png" width="128" height="44" /></a><br />
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            <td width="33%" align="center" valign="top"><div align="center" class="style7">Bacterial <br />
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          <td><h2 class="shadow">Our Sponsors</h2></td>
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            Etch-a-Sketch </div></td>
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            <td width="33%" align="center" valign="top"><div align="center" class="style7">Bacterial <br />
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            Full Adder </div></td>
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            Biobrick Optimizer </div></td>
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          <td><p>&nbsp;</p>
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            <p><a href="https://www.vwrsp.com/" imageanchor="1" rel="nofollow"><img alt="https://www.vwrsp.com/" border="0" height="87" src="http://www.rutgersigem.com/_/rsrc/1316984625451/home/vwr_tag_800.gif?height=87&amp;width=320" width="320" /></a> <a href="www.invitrogen.com"><img border="0" src="http://www.rutgersigem.com/_/rsrc/1314028088138/home/invitrogen.png" /></a></p>
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                <p align="justify">The Etch-a-Sketch project aims to create a lawn of bacteria that can be drawn on with a laser pointer. This seemingly inconsequential task actually presents many interesting engineering challenges. In particular, the bacteria need to be extremely sensitive in order to respond to a short light pulse from a laser, but they still must be &ldquo;selective&rdquo; enough to use in ambient lighting. </p>
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<p><a href="www.qiagen.com"><img border="0" height="170" src="http://www.rutgersigem.com/_/rsrc/1312913435964/home/index.jpg?height=170&amp;width=200" width="200" /></a> <a href="http://www.macrogenusa.com/"><img border="0" src="http://www.rutgersigem.com/_/rsrc/1312913282213/home/Macrogen_logo_medium-300x75.jpg" /></a></p>
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                <p align="justify">We have designed a novel genetic switch that we hope will tackle these problems. If our work proves successful, it will serve as a useful model for future projects that require massive signal amplification. In particular, researchers creating biosensors may find our work very helpful.</p>
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<p>&nbsp;</p></td>
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                <p align="justify">The Full Adder project seeks to create bacteria that can mimic a digital full adder. Since many teams have difficulty creating even something small like a XOR gate, this project would seem nearly impossible. However, we have found that the problem can be greatly simplified if we use a certain simple &ldquo;encoding&rdquo; on the outputs of the full adder. By the end of the summer we hope to demonstrate the world&rsquo;s first (to our knowledge) bacterial full adder. </p>
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                <p align="justify">Our insights may prove useful to any genetic engineer or synthetic biologist working on highly complex systems. If successful, the bacterial full adder may very well become the ancestor to more complicated biological calculators in the future.</p>
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                <p align="justify">A major problem with the current Parts Registry, a library of BioBricks submitted by iGEM teams, is that many parts do not strictly conform to the BioBrick standard which makes certain operations extremely difficult. Rutger's iGEM software team strives to provide a tool to improve the standard parts registry by checking, and if need be modifying, the BioBrick parts. </p>
+
-
                <p align="justify">The basic idea is that before a team submits their new BioBrick, it will run the genetic sequences through MYS!S. MYS!S will output the modified genetic sequence, BioCoder source code, and the lab protocol needed to change the unmodified sequence into the modified sequence  The long term goal of the project is to further the automation of lab protocols by specifying them through algorithms. </p>
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                <p align="justify">The next iteration of the project will focus on a CAD program for BioBricks that will output all the steps necessary to create a genetic circuit in the lab. MYS!S, at first, will be available on Java and Clotho. Later, may expand into iPad and Android.</p>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Rutgers|Home]]
 
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!align="center"|[[Team:Rutgers/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Rutgers Official Team Profile]
 
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!align="center"|[[Team:Rutgers/Project|Project]]
 
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!align="center"|[[Team:Rutgers/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Rutgers/Modeling|Modeling]]
 
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!align="center"|[[Team:Rutgers/Notebook|Notebook]]
 
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!align="center"|[[Team:Rutgers/Safety|Safety]]
 
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!align="center"|[[Team:Rutgers/Attributions|Attributions]]
 
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|}
 

Latest revision as of 13:56, 4 November 2011

Rutgers 2011 iGEM Team: Complex Circuits in Synthetic Biology

Rutgers 2011 iGEM Team: Complex Circuits in Synthetic Biology

RUTGERS iGEM TEAM WIKI

Welcome

For the Rutgers iGEM Team, complex is simple! The Rutgers iGEM Team has designed two complex genetic circuits, Etch-a-Sketch and Full Adder, and created a software tool, MYS!S. The Etch-a-Sketch circuit enables a lawn of bacteria to be drawn on with a laser. This seemingly inconsequential task presents many engineering challenges: the bacteria need to be sensitive in order to respond to a laser pulse, yet selective to use in ambient lighting. The second circuit allows bacteria to emulate a digital full adder. The circuit makes use of individually non-functional split reporters that can reform functional reporters with the help of fused “zipper” domains. In addition to the circuit, we have made easily fuse-able biobricks of these domains in order to facilitate the engineering of more split proteins, which should assist in the creation of logic circuits. MYS!S aims to improve the parts registry by checking and giving directions to modify Biobricks to conform to assembly standards.

Etch-a-Sketch

Full Adder

Mysis

The Etch-a-Sketch project aims to create a lawn of bacteria that can be drawn on with a laser pointer. This seemingly inconsequential task actually presents many interesting engineering challenges. In particular, the bacteria need to be extremely sensitive in order to respond to a short light pulse from a laser, but they still must be “selective” enough to use in ambient lighting.

We have designed a novel genetic switch that we hope will tackle these problems. If our work will serve as a useful model for future projects that require massive signal amplification. In particular, researchers creating biosensors may find our work very helpful.

The Full Adder project seeks to create bacteria that can mimic a digital full adder. This seemingly complex problem can be greatly simplified if we use a certain simple “encoding” on the outputs of the full adder. The full adder incorporates different types of AND gates, including one that incorporates split protein interactions with zipper linker domains. By allowing proteins to be split and reassociated, the zipper domains promise to bring new possibilities to future iGem projects.

Our insights may prove useful to any genetic engineer or synthetic biologist working on highly complex systems. The zipper linker domains create possibilities of new AND interactions, making complexity in future biologic circuits simple. The bacterial full adder may very well become the ancestor to more complicated biological calculators in the future.

A major problem with the current Parts Registry, a library of BioBricks submitted by iGEM teams, is that many parts do not strictly conform to the BioBrick standard which makes certain operations extremely difficult. Rutger's iGEM software team strives to provide a tool to improve the standard parts registry by checking, and if need be modifying, the BioBrick parts.

The basic idea is that before a team submits their new BioBrick, it will run the genetic sequences through MYS!S. MYS!S will output the modified genetic sequence, BioCoder source code, and the lab protocol needed to change the unmodified sequence into the modified sequence The long term goal of the project is to further the automation of lab protocols by specifying them through algorithms.


 

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