Team:Penn State/Research

From 2011.igem.org

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<h1>Experimental Results</h1>
<h1>Experimental Results</h1>
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<p> We are mad scientists! </p>
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<h2>Testing of Or as a Promoter</h2>
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<p>The backbone of the radiation sensor in our project relies on the operating region Or, and the two promoters one on each end of Or. In order to test the function ability of the of the pr promoter we attached a part called mcherry, which has rfp to the promoter. We used the TECAN in our lab to measure the fluorescence of our samples over time. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.</p>
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<img src="https://static.igem.org/mediawiki/2011/b/b5/PSUOrTest.png"/ width="100%">
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Revision as of 02:28, 28 September 2011

PSU iGEM 2011 Home Skip to achievements Skip to experimental results Skip to parts Skip to collaboration
PSU iGEM 2011 Home Wet lab Research Human Practices Results
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Achievements

We did lots of great things! Check it!

Registered Team, had (too much) fun throughout the summer
Attended the Jamboree
At least one submitted and characterized part
Demonstrated part works as expected
Characterized one new part and entered the data in the “Main Page” section of that Part’s/Device’s Registry entry
Worked towards improving the function of an existing BioBrick Part
Helped another team (see our collaboration with NYC wetware below!)
New approach to human practices (Watch our Human Practices Video and learn about our workshop here)

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Experimental Results

Testing of Or as a Promoter

The backbone of the radiation sensor in our project relies on the operating region Or, and the two promoters one on each end of Or. In order to test the function ability of the of the pr promoter we attached a part called mcherry, which has rfp to the promoter. We used the TECAN in our lab to measure the fluorescence of our samples over time. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.

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Parts

Favorites

We need to put these here asap.

List

Dosimeter Design

We will put stuff here

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Collaboration

teamwork!