Team:Peking S/lab/notebook/sr

• Rui Sun's Notebook

summary

In this summer, I mainly focused on the construction of the microbial population density balancer. In addition, I also carried out the assay for orthogonality of positive regulating signaling molecules.

Contents

• July, 2011

• August, 2011

• September, 2011

• October, 2011

July

Mon	Tue	Wed	Thu	Fri	Sat	Sun
-	-	-	-	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31
-	-	-	-	-	-	-

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7.7

ABBREVIATIONS:

Restriction endonuleases:

E=EcoRI X=XbaI S=SpeI P=PstI

17:30 Digestion of B0034(1-2M) with S&P and 4-12I with X&P started.

     Fragment lengths: B0034  around 2000bps
                       4-12I  756 bps
     PCR of terminator-less version of Ptet-rhlI and lacI(I732011)-LVA

     Electroperesis:
     Gel 1 Well 2-4: Marker_4-12I_1-2M
     Gel 2 Well 2-5: Marker_rhlI_rhlI_rhlI
     Gel 3 Well 1-6: Marker_lacI_lacI_ _ _lacI_lacI
     All checked right.

     Gel extraction of the above DNA fragments.

     Ligation of B0034(SP digested)+4-12I(XP digested).

7.8

12:30 Transformation of the ligation product(1-2M+4-12I) finished. 12:30 Gel extraction of PCR product (LacI-LVA) finished.

17:30 PCR for lacI-tag from I732100 started.

19:30 Transformation of 2-10G finished.

19:30 PCR failed. Repeated with the same system &condition and got the right string.

7.9

0 :19 Checked plates.

0 :19 Gel extraction & PCR of lacI-LVA-suffix.

14:00 Digestion of 1-1G and lacI PCR product(second round) with X&P.

14:00 Colony pick of 1-1G plates.

18:30 Electrophoresis for digestion products(1-1G XP fragment;lacI PCR product digested with X&P).

23:45 Ligation of 1-1G and lacI-suffix fragments started.

7.10

7.12

7.13

7.14

7.15

7.16

7.17

7.18

7.19

7.20

7.21

7.22

7.23

7.24

7.25

7.26

7.27

7.28

7.29

7.30

7.31

August

Mon	Tue	Wed	Thu	Fri	Sat	Sun
1	2	3	4	5	6	7
8	9	10	11	12	13	14
15	16	17	18	19	20	21
22	23	24	25	26	27	28
29	30	31	-	-	-	-

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8.1

8.2

8.4

8.5

8.6

8.7

8.8

8.9

8.10

8.11

8.12

8.13

8.14

8.15

8.16

8.17

8.18

8.19

8.20

8.21

8.22

8.23

8.25

8.26

8.27

8.30

8.31

September

Mon	Tue	Wed	Thu	Fri	Sat	Sun
	-	-	1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	-	--

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9.1

9.2

9.3

9.6

9.7

9.8

9.9

9.12

9.13

9.14

9.15

9.16

9.17

9.21

9.22

9.23

9.24

9.25

9.26

9.27

9.28

9.29

9.30

October

Mon	Tue	Wed	Thu	Fri	Sat	Sun
-	-	-	-	1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	-	-	-	-	-	-

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10.1

10.3

10.4

10.5

10.7

10.8

10.9

10.10

10.11

10.12

10.13

10.15

10.16-10.21

10.21-10.25