Team:NYMU-Taipei/chassis

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(Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
(Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
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●ferric quinate solution  2  ml(0.27 g of FeCl<font size=1>3</font> and 0.19 g of quinic acid in 100 ml water)
●ferric quinate solution  2  ml(0.27 g of FeCl<font size=1>3</font> and 0.19 g of quinic acid in 100 ml water)
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  We also refered a paper which added '''Polypeptone''' and '''Yeast extract'''  into MSGM. Which called '''<font color=red>En-rich MSGM</font>'''

Revision as of 15:44, 4 October 2011

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Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

  Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM).  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.


MSGM's ingredients


(In grams per liter)

●succinic acid 0.74 g

●KH2PO4 0.68 g

●sodium nitrate 0.12 g

●sodium thioglycolate 0.1 g

●Wolfe’s vitamin solution 10 ml(stock)

●Wolfe’s mineral solution 5 ml(stock)

●ferric quinate solution 2 ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)


  We also refered a paper which added Polypeptone and Yeast extract  into MSGM. Which called En-rich MSGM

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