Team:NYMU-Taipei/chassis

From 2011.igem.org

(Difference between revisions)
(Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
(Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...)
Line 21: Line 21:
●sodium thioglycolate      0.1 g
●sodium thioglycolate      0.1 g
-
●Wolfe’s vitamin solution  10 ml
+
●Wolfe’s vitamin solution  10 ml(stock)
-
●Wolfe’s mineral solution  5  ml
+
 
 +
●Wolfe’s mineral solution  5  ml(stock)
 +
 

Revision as of 15:14, 4 October 2011

Horizontal Slide Menu



Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

  Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM).  In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.


MSGM's ingredients


(In grams per liter)

●succinic acid 0.74 g

●KH2PO4 0.68 g

●sodium nitrate 0.12 g

●sodium thioglycolate 0.1 g

●Wolfe’s vitamin solution 10 ml(stock)

●Wolfe’s mineral solution 5 ml(stock)

● ● ●