Team:Minnesota/Project

From 2011.igem.org

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Minnesota_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Minnesota_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Minnesota | Team Example]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:Minnesota|<font color="gold">Home</font>]]
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!align="center"|[[Team:Minnesota/Team|<font color="gold">Team</font>]]
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!align="center"|[[Team:Minnesota|Home]]
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!align="center"|[[Team:Minnesota/Project|<font color="gold">Project</font>]]
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!align="center"|[[Team:Minnesota/Team|Team]]
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!align="center"|[[Team:Minnesota/Protocols|<font color="gold">Protocols</font>]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Minnesota Official Team Profile]
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!align="center"|[[Team:Minnesota/Notebook|<font color="gold">Notebook</font>]]
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!align="center"|[[Team:Minnesota/Project|Project]]
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!align="center"|[[Team:Minnesota/Judging|<font color="gold">Judging Criteria</font>]]
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!align="center"|[[Team:Minnesota/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Minnesota/Attributions|<font color="gold">Attributions</font>]]
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!align="center"|[[Team:Minnesota/Modeling|Modeling]]
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!align="center"|[[Team:Minnesota/Safety|<font color="gold">Safety</font>]]
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!align="center"|[[Team:Minnesota/Notebook|Notebook]]
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!align="center"|[[Team:Minnesota/Safety|Safety]]
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== '''''E. coli'' Based Bionanotemplating''' ==
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Our goal is to employ ''E. coli'' to create well defined, solid, two and three dimensional structures by stimulating expression of silicatein on the surface of the cell using light. Once expressed, the silicatein protein (originally from ''Subterities domuncula'') will nucleate silica acid to form rigid structures at the cell surface. By using light as a regulatory expression mechanism, we can create differing two and three dimensional structure by varying the intensity of light incident upon the incubation plate. Intense light exposure will induce maximal expression of surface displayed silicatein in the maximal number of cells in that region whereas a reduction of light exposure should result in a graded response. Furthermore, high light intensity should result in greater penetrance into the incubation medium. The ultimate result of these two factor should be a definable rigid structure.
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== Surface Displayed Silicatein ==
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Our surface display team intends to use either an ompA or ice nucleation protein fusion chimera to display silicatein on the surface of the ''E. coli'' cell. Both ompA and ice nucleation protein can serve as a suitable carrier for other proteins which need to be localized to the exterior of the cell. The silicatein gene will be fused to the carrier protein using recombinant genetic methods. This fusion gene will then be expressed in living cells under stringent genetic regulation and the kinds of materials that can be nucleated will be observed.
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== '''Overall project''' ==
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== System Regulation ==
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Your abstract
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Our regulatory team has decided to reconstitute the light induction system or Coliroid which was the work of the 2004 joint University of Texas at Austin and UCSF iGEM team and is described at http://partsregistry.org/Coliroid. The Coliroid is a two component response mechanism which is composed of three genes. First, heme is used to create a phycocyanobilin by two genes isolated from ''Synechocystis'', pcyA and ho1. This phycocyanobilin then associates with a chimeric light receptor, Cph8 which is the fusion product of Cph1 and Envz-OmpR. Stimulation with red light inhibits autophosporylation of the receptor which can be leveraged to activate expression of our surface displayed silicatein chimera.
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== Project Details==
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== Results ==
== Results ==
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Revision as of 11:57, 15 July 2011

Mnlogo.jpg
Home Team Project Protocols Notebook Judging Criteria Attributions Safety

E. coli Based Bionanotemplating

Our goal is to employ E. coli to create well defined, solid, two and three dimensional structures by stimulating expression of silicatein on the surface of the cell using light. Once expressed, the silicatein protein (originally from Subterities domuncula) will nucleate silica acid to form rigid structures at the cell surface. By using light as a regulatory expression mechanism, we can create differing two and three dimensional structure by varying the intensity of light incident upon the incubation plate. Intense light exposure will induce maximal expression of surface displayed silicatein in the maximal number of cells in that region whereas a reduction of light exposure should result in a graded response. Furthermore, high light intensity should result in greater penetrance into the incubation medium. The ultimate result of these two factor should be a definable rigid structure.

Surface Displayed Silicatein

Our surface display team intends to use either an ompA or ice nucleation protein fusion chimera to display silicatein on the surface of the E. coli cell. Both ompA and ice nucleation protein can serve as a suitable carrier for other proteins which need to be localized to the exterior of the cell. The silicatein gene will be fused to the carrier protein using recombinant genetic methods. This fusion gene will then be expressed in living cells under stringent genetic regulation and the kinds of materials that can be nucleated will be observed.

System Regulation

Our regulatory team has decided to reconstitute the light induction system or Coliroid which was the work of the 2004 joint University of Texas at Austin and UCSF iGEM team and is described at http://partsregistry.org/Coliroid. The Coliroid is a two component response mechanism which is composed of three genes. First, heme is used to create a phycocyanobilin by two genes isolated from Synechocystis, pcyA and ho1. This phycocyanobilin then associates with a chimeric light receptor, Cph8 which is the fusion product of Cph1 and Envz-OmpR. Stimulation with red light inhibits autophosporylation of the receptor which can be leveraged to activate expression of our surface displayed silicatein chimera.


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