Team:Lyon-INSA-ENS/Realisation/Protocols/Fermentas

From 2011.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 2: Line 2:
{{Lyon-INSA-ENS/blocStyle}}
{{Lyon-INSA-ENS/blocStyle}}
{{INSA-Lyon/styletestaurelie}}
{{INSA-Lyon/styletestaurelie}}
-
{{Lyon-INSA-ENS/menuhorizontalRealisation}}
+
{{Lyon-INSA-ENS/menuhorizontal}}
-
{{Lyon-INSA-ENS/menuRealisationProtocoles}}
+
<!--{{Lyon-INSA-ENS/menuNotebookProtocoles}}-->
 +
{{Lyon-INSA-ENS/menuNotebookVertical|Protocols = actif}}
<html>
<html>

Latest revision as of 10:19, 10 January 2012





Fermentas digestion





This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.





Procedure



1. In an eppendorf tube, add the following solutions in any order :
-250 ng DNA ( if the concentration is unknown, use 5µL )
-2.5µL (1X) or 5µL (2X) 10X tango buffer ( this depends on the enzymes used )
-water to get a volume of 25µL after addition of the enzymes


2. Add 1µL of each one of the desired enzymes.


3. Incubate at 37°C for 1h30


4. Incubate at 70°C for 10 mn to inactivate the restriction enzymes.








ENS assystem Biomérieux INSA INSA