Team:Lyon-INSA-ENS/Realisation/Protocols

From 2011.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 7: Line 7:
<html>
<html>
 +
 +
<div style="float : left; margin-top : -10px; margin-left : -200px">
 +
  <a href="https://2011.igem.org/Main_Page" >
 +
      <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
 +
  </a>
 +
</div>
<body>
<body>
Line 139: Line 145:
     </p>
     </p>
      
      
-
<br><br><br><br><br><br><br><br><br><br><br><br><br>
+
<br><br><br><br><br><br><br>
 +
 
 +
    <p>
 +
              <a  href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/BeforeStarting"/><font color="grey"><b>Before Starting</b></font></a>
 +
              <a  style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Notebook/Week1"/><font color="grey"><b>First Week</b></font></a>
 +
              <br/>
 +
    </p>
 +
 
 +
<br><br><br>
</div>
</div>
</body>
</body>

Latest revision as of 16:08, 20 September 2011







General culture conditions






Antibiotics were used at the following concentrations :

Ampicilin (Amp) : 100µg/mL
Chloramphenicol (Cm) : 20µg/mL
Spectinomycin (Spc) : 100µg/mL
Kanamycin (Kan) : 30µg/mL
The solvent is a 70/30 (v/v) water/ethanol mix for chloramphenicol, other antibiotics were dissolved in water. All volumes mentionned are meant for a 100X mother solution.

The composition of the different media we have used is the following :

LB : 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl for 1L of liquid LB. Solid LB for plates is made by adding agar to reach a concentration of 1.5%.
M63G : 100/1/1 (v/v) of M63, glucose 20% and LB
LB/2 : 50/50 (v/v) mix of LB and water








ENS assystem Biomérieux INSA INSA