Team:Lethbridge/Notebook/Lab Work/Group1

From 2011.igem.org

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=April=
=April=
==April 1, 2011==
==April 1, 2011==
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<br>- Confirmation Gel of I1343, B0034, C0040 & B0015</br>
+
<i> Confirmation Gel of I1343, B0034, C0040 & B0015</i>
-
<br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4.</br>
+
<br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4.
-
<br>- A gel was made following the 1% Agarose Gel Protocol (100mL).</br>
+
<br>- A gel was made following the 1% Agarose Gel Protocol (100mL).
-
<br>- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder.</br>
+
<br>- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder.
-
<br>- The gel ran at 120V for 60 minutes.</br>
+
<br>- The gel ran at 120V for 60 minutes.
-
<br>- The gel was then stained in Ethidium Bromide for 10 minutes.</br>
+
<br>- The gel was then stained in Ethidium Bromide for 10 minutes.
-
<br>- Results: The parts were confirmed with the correct sizes.</br>
+
<br>- Results: The parts were confirmed with the correct sizes.
==April 2, 2011==
==April 2, 2011==
-
<br>- Assembly of I1343 & B0034 and C0040 & B0015</br>
+
<i>Assembly of I1343 & B0034 and C0040 & B0015</i>
-
<br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.</br>
+
<br>- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.
-
<br>- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1. </br>  
+
<br>- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1.   
-
<br>- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1. </br>
+
<br>- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1.  
-
<br>- Both ligations were transformed following the Transformation Protocol 1.</br>
+
<br>- Both ligations were transformed following the Transformation Protocol 1.
-
 
+
==April 21, 2011==
==April 21, 2011==
==April 23, 2011==
==April 23, 2011==
-
==April 27, 2011 ==
+
==April 27, 2011==
==April 28, 2011==
==April 28, 2011==

Revision as of 19:03, 15 September 2011


Contents

April

April 1, 2011

Confirmation Gel of I1343, B0034, C0040 & B0015
- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 4.
- A gel was made following the 1% Agarose Gel Protocol (100mL).
- The restrictions were loaded onto the gel with a 1kb ladder and 100bp ladder.
- The gel ran at 120V for 60 minutes.
- The gel was then stained in Ethidium Bromide for 10 minutes.
- Results: The parts were confirmed with the correct sizes.

April 2, 2011

Assembly of I1343 & B0034 and C0040 & B0015
- Each of I1343, B0034, C0040 & B0015 were restricted following the Restriction Protocol 1 where all odd parts are upstream and even parts are downstream.
- I1343 & B0034 were ligated together in pSB1A3 following the Ligation Protocol 1.
- C0040 & B0015 were ligated together in pSB1A3 following the Ligation Protocol 1.
- Both ligations were transformed following the Transformation Protocol 1.

April 21, 2011

April 23, 2011

April 27, 2011

==April 28, 2011==
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