Team:Kyoto/Digestion

From 2011.igem.org

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Revision as of 12:35, 22 September 2011

Contents

Project Digestion

Introduction

Streptomyces is a kind of prokaryotic bacteria which decompose bodies in nature. We extract protease and chitinase genes from this bacterium and introduce into Escherichia coli. Secretion-signal sequences are included in these genes so that the proteins coded by them will go out without occurring cell lysis. After assembling all genes, we examined the activity of these two enzymes in both of qualitative and quantitative ways.

Method

Construction

Assay

Serine Protease

Result

Discussion