Team:Imperial College London/Templates/Kill

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Module 3: Gene Guard

Containment is a serious issue concerning the release of GMOs into the environment. To prevent horizontal gene transfer of the genes we have expressed in our chassis, we have developed a toxin/anti-toxin system based on the genes encoding holin, anti-holin and endolysin. We are designing our bacteria to contain anti-holin (the anti-toxin) within its genome and holin and endolysin (the toxin) on the plasmid with the Phyto-Route and Auxin Xpress genes. In the event of horizontal gene transfer to a soil bacterium, holin and endolysin would be transferred without anti-holin, effectively killing the recipient cell.



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