Team:Imperial College London/Project/Arabidopsis/Overview

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-<h1>Arabidopsis: our model soil erosion-preventing plant</h1>
-Arabidopsis thaliana is a common plant model organism. It belongs to the mustard family and fulfils many important requirements for model organisms. As such, its genome has been almost completely sequenced and replicates quickly, producing a large number of seeds. It is easily transformed and many different mutant strains have been constructed to study different aspects (1). While Arabidopsis may not represent plant populations naturally occurring in arid areas threatened by desertification, it is a handy model organism we will be using to study the effect of auxin on roots, observe chemotaxis towards them and look at uptake of bacteria into the roots. <br><br>
-Arabidopsis will be used as our model plant for plant-bacteria interactions. We will be using wild type (wt) plants do replicate the results described by Paungfoo-Lonhienne et al. (2010) and demonstrate that we can get plant roots to take up bacteria. Paungfoo-Lonhienne et al. demonstrated that plants actively break down their cell wall to take up bacteria. The bacteria are then digested and supply the plants with nutrients (2).<br><br>
-In later experiments, we will be using Arabidopsis to look at the uptake of our engineered bacteria into the plants.<br><br>
-We will use DR5:GFP and DR5:3XVENUS plants that respond to auxin by expression of GFP and YFP, respectively, to look at the plant response to synthetic auxin and later bacteria-secreted auxin. Initially, we will be supplying the plants with synthetic auxin and observe the differences in growth and (root) morphology due to differential concentrations of the hormone.<br><br>
-(1) <a href="http://www.nih.gov/science/models/arabidopsis/index.html">http://www.nih.gov/science/models/arabidopsis/index.html</a><br>
-(2) <a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011915">Paungfoo-Lonhienne et al. (2010) Turning the table: Plants consume microbes as a source of nutrients. PLoS ONE 5:1-11.</a>
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Team:Imperial College London/Project/Arabidopsis/Overview

From 2011.igem.org

Revision as of 10:42, 12 August 2011