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Team:Imperial College London/Project/Arabidopsis/Notebook
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Revision as of 17:50, 3 August 2011 by RebekkaBauer (Talk | contribs)
Wednesday, 20 July 2011
Two cultures of wt Arabidopsis thaliana Columbia strain have been set up. Phytogel cultures were set up on Saturday, 15 July and liquid cultures were set up on Wednesday, 20 July.Wednesday, 27 July 2011
Preparing culture and preparing the Venus seedlingThursday, 28 July 2011
Growing the Venus in 5 of the prepared 100 ml mediaFriday, 29 July 2011
Start modelling the effect of root branching by auxinJames field preparing GFP bacteria, as supplied by Dr Tom Ellis's lab
Monday, 1 August 2011
Seedling, preparing media and grow more Venus and GFP in 5 x 2 of 100 ml mediaStocking the GFP bacterial culture in glycerol
Start growing bacterial culture in 188 LB media overnight for plant infection tomorrow
Wednesday, 3 August 2011
Today, we prepared new phytogel cultures as the ones we set up previously were getting too poor in nutrients.We met with Dr Martin Spitaler who advised us on how to prepare samples for the confocal microscopy we will be doing on Friday. The confocal microscopy will focus on imaging GFP expressing bacteria inside Arabidopsis roots to show that uptake of the bacteria takes place. Staining of wt roots with DiD, a lipophilic dye that stains the plant membranes and does not interfere with the absorption or emission spectra of GFP and Dendra, was unsuccessful. However,
