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Team:Imperial College London/Project/Arabidopsis/Notebook
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<h1>Wednesday, 20 July 2011</h1> | <h1>Wednesday, 20 July 2011</h1> | ||
Two cultures of wt Arabidopsis thaliana Columbia strain have been set up. Phytogel cultures were set up on Saturday, 15 July and liquid cultures were set up on Wednesday, 20 July.<br> | Two cultures of wt Arabidopsis thaliana Columbia strain have been set up. Phytogel cultures were set up on Saturday, 15 July and liquid cultures were set up on Wednesday, 20 July.<br> | ||
| + | 8 of phytogels are seeded with WT arabidopsis at 1 cm below 1 rim of the plate. Each seed is separated to 1 cm. The phytogel is put under light on the shelves in the planting room to allow the space for roots to grow downwards where we grow the roots for 2 weeks. | ||
<h1>Wednesday, 27 July 2011</h1> | <h1>Wednesday, 27 July 2011</h1> | ||
| - | Preparing | + | Preparing MS liquid media and preparing the 2 eppendorf of Venus seedling using the described protocol<br> |
<h1>Thursday, 28 July 2011</h1> | <h1>Thursday, 28 July 2011</h1> | ||
| - | Growing | + | Growing each 20 seeds of Venus in 5 of the prepared 100 ml media by pipetting the seed and transfer the seeds into the media flask under fume cupboard. The flasks are put into the shakers under the light described in the protocol<br> |
<h1>Friday, 29 July 2011</h1> | <h1>Friday, 29 July 2011</h1> | ||
| - | Start modelling the effect of root branching by auxin <br> | + | Start modelling the effect of root branching by auxin. Sending the article involving auxin branching to Nina. <br> |
James field preparing GFP bacteria, as supplied by Dr Tom Ellis's lab <br> | James field preparing GFP bacteria, as supplied by Dr Tom Ellis's lab <br> | ||
<h1>Monday, 1 August 2011</h1> | <h1>Monday, 1 August 2011</h1> | ||
Revision as of 23:30, 3 August 2011
Wednesday, 20 July 2011
Two cultures of wt Arabidopsis thaliana Columbia strain have been set up. Phytogel cultures were set up on Saturday, 15 July and liquid cultures were set up on Wednesday, 20 July.8 of phytogels are seeded with WT arabidopsis at 1 cm below 1 rim of the plate. Each seed is separated to 1 cm. The phytogel is put under light on the shelves in the planting room to allow the space for roots to grow downwards where we grow the roots for 2 weeks.
Wednesday, 27 July 2011
Preparing MS liquid media and preparing the 2 eppendorf of Venus seedling using the described protocolThursday, 28 July 2011
Growing each 20 seeds of Venus in 5 of the prepared 100 ml media by pipetting the seed and transfer the seeds into the media flask under fume cupboard. The flasks are put into the shakers under the light described in the protocolFriday, 29 July 2011
Start modelling the effect of root branching by auxin. Sending the article involving auxin branching to Nina.James field preparing GFP bacteria, as supplied by Dr Tom Ellis's lab
Monday, 1 August 2011
Seedling, preparing media and grow more Venus and GFP in 5 x 2 of 100 ml mediaStocking the GFP bacterial culture in glycerol
Start growing bacterial culture in 188 LB media overnight for plant infection tomorrow
Wednesday, 3 August 2011
Today, we prepared new phytogel cultures as the ones we set up previously were getting too poor in nutrients.We met with Dr Martin Spitaler who advised us on how to prepare samples for the confocal microscopy we will be doing on Friday. The confocal microscopy will focus on imaging GFP expressing bacteria inside Arabidopsis roots to show that uptake of the bacteria takes place. Staining of wt roots with DiD, a lipophilic dye that stains the plant membranes and does not interfere with the absorption or emission spectra of GFP and Dendra, was unsuccessful. However,
